Single-Cell Multi-Omics (CITE-seq / REAP-seq) Service
Single-cell multi-omics profiling, incorporating techniques like CITE-seq and REAP-seq, enables the simultaneous measurement of both the transcriptome and proteome at the single-cell level. This dual approach enhances the understanding of cellular heterogeneity and the relationships between gene expression and surface protein markers in a variety of biological contexts.
Our service offers high-throughput, precise multi-modal analysis, providing comprehensive insights into cell function, identity, and behavior, particularly valuable for applications in immunology, cancer research, and stem cell biology.
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- Workflow
- Our service
- Applications
- Why choose
- FAQs
- Sample preparation
Workflow
The process begins by staining a single-cell suspension with DNA-barcoded antibodies targeting specific surface proteins. These labeled cells are then microfluidically isolated into droplets alongside barcoded beads, which simultaneously capture both the cell's native mRNA and the antibody tags. After amplification, the genetic material is separated into distinct gene expression and protein (ADT) libraries and sequenced via NGS. Finally, bioinformatics pipelines use the shared barcodes to integrate the RNA and protein data, mapping both back to individual cells to generate a comprehensive multi-omic profile.
What We Provide
- High-quality single-cell RNA and protein expression data
- Detailed analysis using state-of-the-art sequencing technologies
- Expertise in assay design and data interpretation
- Custom antibody and gene panel development tailored to specific research objectives
Key Applications
- Immunology: Characterizing immune cell subsets and their responses to various stimuli.
- Cancer Research: Investigating tumor microenvironments and identifying potential biomarkers.
- Stem Cell Biology: Studying differentiation processes and cellular identity.
- Developmental Biology: Mapping lineage and gene expression in complex tissues.
Why Choose Us?
- Simultaneous Transcriptome and Proteome Profiling: Measure RNA and protein expression in single cells, increasing the depth of cellular characterization.
- High-Resolution Data: Obtain high-dimensional data to explore intricate cellular states and developmental trajectories.
- Multiple Platforms Supported: Compatible with CITE-seq (Cellular Indexing of Transcriptomes and Epitopes by sequencing) and REAP-seq (RNA Expression and Protein sequencing) techniques for flexible, scalable solutions.
- Customizable Panel Design: Tailor the antibody and gene panels to suit your specific research needs.
FAQs
What is the difference between CITE-seq and REAP-seq?
Both CITE-seq and REAP-seq simultaneously measure single-cell RNA and cell-surface proteins. The primary difference is how the DNA barcode is attached to the antibody: CITE-seq uses a non-covalent linkage (streptavidin-biotin interaction), whereas REAP-seq uses a direct, covalent chemical linkage.
What types of proteins can be detected?
The detection is customizable based on the antibodies used, enabling the study of a broad range of surface proteins.
Learn about other Q&A.
Sample Submission Guidelines
| Sample Type | Requirements |
|---|---|
| Fresh or Frozen Tissue | 50-100 mg |
| Single-Cell Suspensions | >1 million viable cells per sample |
| Cultured Cells | >500,000 cells per sample |
| Human/Mouse Blood | >300,000 viable cells |
| PBMCs | >300,000 cells per sample |
Note: Please consult with our experts before preparing your samples to determine the best approach for your project.
