NADP+ is the abbreviation of Nicotinamide adenine dinucleotide phosphate, and is is a cofactor used in anabolic reactions, such as lipid and nucleic acid synthesis. NADPH is the reduced form of NADP+. NADPH is produced in the last step of photosynthesis ferredoxin-NADP+ reductase in the photosynthetic organisms. In animals and other non-photosynthetic organisms, the major source of NADPH is the pentose phosphate pathway, which, generates NADPH and pentoses (5-carbon sugars) as well as Ribose 5-phosphate.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of NADP+/NADPH levels in various samples, including Animals, Plants, Microorganisms, Cells and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of NADP (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of NADP measurement, which enables us to analyze of NADP levels in vitro and in vivo.
The regeneration of reduced glutathione, which is abbreviated as GSH in oxidation-reduction requires reducing equivalents provided by NADPH. The biochemical process can protect the cells from the toxicity of reactive oxygen species, which is abbreviated as ROS. NADPH is also used for lipid synthesis,cholesterol synthesis, and fatty acid chain elongation. The NADPH system is also involved in the generating free radicals in immune cells. These radicals are involved in the biological process named respiratory burst, which is the rapid release of reactive oxygen species (superoxide radical and hydrogen peroxide) from different types of cells in order to destroy pathogens in the immune system. NADPH also can be used as reducing equivalents for cytochrome P450 hydroxylation of aromatic compounds, steroids, alcohols, and drugs.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
NADP+/NADPH Analysis Service at Creative Proteomics supports your research in NADP+/NADPH Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Animals, Plants, Microorganisms, Cells and more
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of NADP (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of NADP measurement, which enables us to analyze of NADP levels in vitro and in vivo.
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