Glycerolipids Analysis Service


The two major classes of complex lipids in  biological materials are glycerolipids and sphingolipids, which are substituted  derivatives of glycerol and of sphingosine or a related long-chain base  respectively. We have developed approaches involving ESI-MS or Q-TOF MS to  characterize both glycerolipids and sphingolipids. We are able to  characterization of the glycerolipids of pathogenic microorganisms as well as  in mammals.

Glycerolipids analysis service

Glycerolipids analyses are conducted on  GC/MS, LC/MS, and LC/hybrid MS platforms at Creative Proteomics. We have  pioneered the use of contemporary chromatography systems and high resolution  mass spectrometers to identify and characterize several lipid classes. These  resources and expertise have advanced our understanding of at least 2 major  complex lipid classes that exist in biological organisms: glycerolipids and  sphingolipids.

The simplest glycerolipids contain only  fatty acid substituents esterified to the glycerol backbone, and we have  developed positive ion ESI/MS/MS methods for charactering both neutral acyl  glycerols and fatty acids. Glycerolipids with ethanolamine, serine, inositol,  or glycerol head-groups are in general more readily characterized by negative  ion ESI/MSn by collisionally activated dissociation (CAD) of [M – H]- ions.

Sample  Preparation

A variety of sample preparations can be  performed. Liquid and liquid-liquid extractions are the most common. A variety  of solid phase extraction (SPE) protocols are used in the lab. Sample  homogenization can be done by sonication or with a bead homogenizer. And large  sample numbers can be prepared by robotic equipment.

Glycerolipids could monitored in This  Service

Ordering Procedure:

Ordering Procedure

With integrated set of separation, characterization,  identification and quantification systems featured with  excellent robustness & reproducibility, high and ultra-sensitivity,  Creative Proteomics provides reliable, rapid and  cost-effective Lipidomics services.


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