The two major classes of complex lipids in biological materials are glycerolipids and sphingolipids, which are substituted derivatives of glycerol and of sphingosine or a related long-chain base respectively. We have developed approaches involving ESI-MS or Q-TOF MS to characterize both glycerolipids and sphingolipids. We are able to characterization of the glycerolipids of pathogenic microorganisms as well as in mammals.
Glycerolipids analyses are conducted on GC/MS, LC/MS, and LC/hybrid MS platforms at Creative Proteomics. We have pioneered the use of contemporary chromatography systems and high resolution mass spectrometers to identify and characterize several lipid classes. These resources and expertise have advanced our understanding of at least 2 major complex lipid classes that exist in biological organisms: glycerolipids and sphingolipids.
The simplest glycerolipids contain only fatty acid substituents esterified to the glycerol backbone, and we have developed positive ion ESI/MS/MS methods for charactering both neutral acyl glycerols and fatty acids. Glycerolipids with ethanolamine, serine, inositol, or glycerol head-groups are in general more readily characterized by negative ion ESI/MSn by collisionally activated dissociation (CAD) of [M – H]- ions.
A variety of sample preparations can be performed. Liquid and liquid-liquid extractions are the most common. A variety of solid phase extraction (SPE) protocols are used in the lab. Sample homogenization can be done by sonication or with a bead homogenizer. And large sample numbers can be prepared by robotic equipment.
Glycerolipids could monitored in This Service
|3||Monoacyl glycerols, etc.|
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.
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