Currently, proteomics is as important as genomics.
As one of the core contents of the "post-genome" era, proteomics directly and comprehensively analyzes the protein abundance or PTMs level of samples, and comprehends regulatory mechanisms such as protein function and interaction relationship that cannot be determined by genomics and transcriptomics. Importantly, proteins are direct products of genes; one gene sequence may produce two or more protein products. Even under different conditions or at different period, the cellular proteome may be completely different. Therefore, proteomics research is of great value for pathology, toxicology and pharmacokinetics investigation. The advancement of proteomics technologies has significantly enhanced the opportunities for identifying diagnostic markers of diseases and screening potential drug targets.
What is proteomics?
The field of proteomics involves a comprehensive and systematic investigation into the complete repertoire of proteins expressed by organisms or cells, encompassing information on protein expression abundances, variations and degradation, post-translational modifications (PTMs), as well as their interacting partners and networks. By conducting qualitative, quantitative, molecular function analysis, pathway analysis and protein interaction analysis of the proteome, proteomics can better reveal biological functions, mechanisms, prediction of upstream and downstream changes in proteins and disease markers for diagnosis. Besides, proteomics can overcome the uncertainty of nucleic acid level prediction and reflect translation results. Importantly, MS-based proteomics has undergone rapid development and significant maturation over the past few decades that collectively contribute greatly to our understanding of protein functions and biological systems.
The main contents of proteomics research
1) Structural proteomics;
2) Discovery proteomics;
3) Functional proteomics;
4) Differential proteomics;
5) Spatial proteomics.
In discovery proteomics (untargeted proteomics), there are mainly two distinct strategies for analyzing the proteome: bottom-up and top-down The bottom-up approach involves initially subjecting a crude protein mixture to protease digestion, such trypsin, lys-C, followed by separation through liquid chromatography and subsequent analysis using tandem mass spectrometry (LC-MS/MS). While in the top-down method, intact proteins are characterized directly using MS without prior proteolysis. Different methods have their own application scenarios, and both methods have been greatly improved with the development of proteomics, although bottom-up analysis remains the most common proteomics technology.
Nowadays, proteomics research is widely applied in many scientific fields, such as:
1) Diabetes research;
3) Diagnostic biomarker discovery;
7) Toxicological testing;
9) Antibody profiling for study and treatment disease.
2) Cancer research;
4) Plant and animal phenotyping;
6) Drug discovery and development;
8) Structural biology;
Our proteomics services
In Creative Proteomics, we will professionally design for your project as figure 1. The classical process is: 1) Project design; 2) Protein fractionation; 3) Peptide fractionation; 4) Mass spectrometry; 5) Data identification and analysis; 6) Quantification.
Figure 1. A typical MS-based proteomic workflow.
The high-throughput proteomics approaches employed by Creative Proteomics are powerful tools that facilitate the identification and quantification of thousands, or even millions, of proteins from a single analysis for large-scale protein profiling. Importantly, equipped with a highly experienced technical team and a senior bioinformatics data processing team, we possess the capability to handle diverse sample types and provide comprehensive investigations, encompassing whole proteome profiling, subcellular proteomics analysis, PTMs characterization, protein-protein interaction assessment, and monitoring of protein turnover dynamics. We are also professional in small protein (length<100), or peptidomics investigation.
- Professional detection and analysis capability: Experienced research team, strict and matured techniques.
- Breadth: we could skillfully deal with protein samples derived from a wide range of sources, encompassing animal and plant tissues, bacteria, blood, membrane proteins, nuclear proteins, secreted proteins, etc.
- High stability and reproducible: Obtain consistent and reproducible inter- and intra- assay results for data analysis.
- High specificity and accuracy: Skillful quantification proteomics techniques, and PTMs enrichment methods.
- High resolution and sensitivity: Triple TOF 5600, Q-Exactive, Q-Exactive HF, Orbitrap Fusion™ Tribrid.
How to place an order
At Creative Proteomics, experienced proteomics research team, strict quality control system, together with ultra-high resolution detection system and professional data pre-processing and analysis capability, ensure reliable and accurate data. Please feel free to contact us by email to discuss your specific needs. Our customer service representatives are available 24 hours a day, from Monday to Sunday.