Fatty Acid Metabolism Analysis Service

Fatty Acid Metabolism Analysis Service

A fatty acid is composed of a  terminal carboxylate group and a long hydrocarbon chain. Fatty acids exert four  major physiological roles in the organism. Firstly, fatty acids serve as  building blocks of amphipathic molecules such as phospholipids and glycolipids,  which are important components of biological membranes. Secondly, fatty acids  can target many proteins to membrane locations by covalently attached to the  targeted proteins. Thirdly, stored as triacylglycerols, uncharged  esters of fatty acids with glycerol, fatty acids are the preferred fuel  molecules for the heart and the preferred energy source for skeletal muscle at  the time of prolonged exertion. Triacylglycerols are catabolized into free  fatty acid and glycerol. In the liver and peripheral, through β-oxidation, free  fatty acid is metabolized into acetyl CoA while glycerol is used for  gluconeogenesis or triglyceride regeneration. Finally, fatty acid derivatives  act as intracellular messengers and hormones.

The degradation and synthesis of fatty  acid are rather simple and they are the reverse of each other. Through the  degradation pathway, the aliphatic compound fatty acid is converted to a series  of activated acetyl units to enter citric acid cycle and offer energy. Through  oxidation, fatty acid is activated and a double bond is introduced. By  introducing oxygen, the double bond is hydrated. The alcohol is then oxidized  and a ketone is formed. Finally, coenzyme A cleaves the four carbons  fragment and acetyl CoA and a fatty acid with chains two carbons shorter are  generated. If the fatty acid is saturated and has an even number of carbon  atoms, the process will continue till the fatty acid is completely converted to  acetyl CoA units. For each two carbons in the fatty acid, 5 ATPs is  generated during the oxidation to acetyl CoA and another 12 ATPs through the  coenzyme oxidation. Therefore, fatty acid is a rather potent energy store  source.

The synthesis of fatty acid is  essentially the reverse of this degradation process. The synthesis of fatty  acids starts from activated acyl group and malonyl units. The malonyl unit  reacts with the acetyl unit and a four-carbon fragment is generated. Exactly  the opposite of degradation, the fragment is then reduced, dehydrated, and  reduced again, the carbonyl group is then brought to the level of a methylene  group and the butyryl CoA is then formed at the same time. Butyryl unit  reacts with another activated malonyl group and this process continues till a  C18 fatty acid is synthesized.

Since fatty acids metabolism plays a  crucial role in large number of disorders like cardiovascular disease and  certain types of cancer, the detection and quantification of these compounds  are of great importance. Since the endogenous fatty acids metabolites are of  rather low concentration, sensitive and specific analytical methods are  required for the reliable quantification of these compounds. HPLC-MS/MS has  emerged as one of the main techniques used for fatty acids metabolites  quantification. Creative Proteomics has established sensitive, reliable, and  accurate LC-MS/MS method for quantification of fatty acids metabolites.



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Fatty Acids Metabolites Quantified in This Service

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With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective fatty acids metabolism targeted lipidomics services.

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