MS/MS based Sequencing of Peptides

Analytical characterization of purified proteins, in particular recombinant proteins for drug discovery and development, requires confirmation of protein sequence. N- and C-terminal Protein Sequencing can be used for verification of protein terminals to investigate protein degradation products and enzyme cleavage specificity.

Except the conventional cyclic Edman degradation for N-terminus amino acid sequencing, Creative Proteomics present a powerful mass spectrometry method (MALDI ISD) that provides partial sequence information of targeted proteins with up to 20-50 amino acid residues from both the N-terminal and C-terminal in one single analysis. Even N-terminally modified and blocked proteins (acetylated, pyroglutamate, PEGylated) can be sequenced fast.

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