Flow Cytometry Service
Creative Proteomics provides advanced flow cytometry solutions for precise cell sorting, enabling accurate, high-purity analysis of complex cell populations in research applications.
- Creative Proteomics offers high-performance flow cytometry solutions.
- Advanced optics and superior fluorescence detection enable rapid, high-purity sorting of complexcell populations.
- Multiparameter analysis for in-depth cellular function exploration.
- Low-shear design maintains cell viability during sorting.
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What Is Flow Cytometry?
Flow cytometry is a versatile and high-throughput technology that allows for the rapid, quantitative analysis of individual cells based on their physical and biochemical properties. In this technique, cells in suspension pass through a laser beam where they are interrogated for a variety of parameters. Light scatter measurements provide information about cell size (forward scatter) and granularity (side scatter), while fluorescence detectors capture data on the expression of specific surface or intracellular proteins tagged with fluorescent probes. The technology's core strength lies in its ability to measure multiple parameters simultaneously at the single-cell level, offering both high sensitivity and throughput, with the capability to analyze tens of thousands of cells per second. This makes flow cytometry a powerful tool in a range of biological and clinical applications, including immune cell phenotyping, stem cell research, tumor microenvironment analysis, and biomarker discovery. In addition, the ability to sort cells based on specific characteristics enhances its use in applications like single-cell omics studies and therapeutic monitoring. Combined with fluorescent antibodies, nucleic acid probes, or functional dyes, flow cytometry is an essential platform for advancing research in immunotherapy, drug development, and personalized medicine, enabling researchers and clinicians to gain detailed, real-time insights into cellular behavior and disease mechanisms. Its precision and speed make it an indispensable tool for dissecting biological complexity and driving innovations in disease diagnostics and treatment strategies.
In modern biomedical research and clinical practice, flow cytometry serves not only as a fundamental tool for quantitative cellular analysis but also as a key platform for cell sorting, single-cell omics studies, and immunotherapy evaluation. Its precision and throughput make it an indispensable technology for decoding biological complexity and advancing personalized medicine.
Why Choose Flow Cytometry?
- Multiparametric Analysis
Simultaneously measures multiple cellular features — including size, granularity, and surface or intracellular protein expression — enabling comprehensive, high-dimensional characterization of cell populations. - High Throughput and Speed
Capable of analyzing tens of thousands of cells per second, providing rapid and statistically robust data acquisition for large-scale studies. - Single-Cell Resolution
Analyzes cells individually, revealing population heterogeneity and supporting precision medicine and single-cell omics research. The techniques used in this research are more versatile. - Quantitative and Highly Sensitive
Fluorescence intensity offers precise quantification of target molecule expression with high sensitivity and excellent reproducibility. - Broad Application Spectrum
Widely used in immunophenotyping, cancer biology, stem cell differentiation, drug screening, vaccine development, and biomarker discovery. - Cell Sorting Capability
Enables isolation and recovery of specific cell subsets for downstream culture, molecular analysis, or functional assays.
Flow Cytometry Service at Creative Proteomics
Creative Proteomics provides comprehensive support for flow cytometry–based research and cell analysis. Our advanced flow cytometry platform combines high-resolution optics, multi-laser excitation, and high-sensitivity fluorescence detection, enabling accurate multiparameter analysis at the single-cell level.
With extensive expertise in cellular phenotyping and functional analysis, Creative Proteomics offers customized flow cytometry services covering a broad range of applications. Our capabilities include cell cycle analysis, apoptosis detection, immune cell typing, surface antigen identification, intracellular calcium detection, mitochondrial function assessment, and high-purity cell sorting. These services empower researchers to gain detailed insight into cellular behavior, signaling pathways, and immune responses.
Our flow cytometry laboratory provides comprehensive support from sample preparation and experimental design to data acquisition and bioinformatic analysis. Our high-speed cell sorters allow precise isolation of defined subpopulations for downstream molecular research, cell culture, or therapeutic development.
As single-cell technologies continue to shape the future of life sciences, Creative Proteomics is dedicated to delivering accurate, reproducible, and customized flow cytometry solutions that meet the diverse needs of academic, clinical, and biopharmaceutical research worldwide. Currently, Creative Proteomics has undertaken thousands of flow cytometry projects, including:
- Flow cytometry used for cell cycle analysis, DNA ploidy analysis, and quantitative analysis to detect oncogene protein products, drug resistance proteins, and apoptosis.
- Flow cytometry used for absolute counting of lymphocyte subsets, hematopoietic stem cells, reticulocytes, platelets, etc. in peripheral blood or bone marrow.
- Flow cytometry for detection of specific nucleic acid sequences or specific gene abnormalities in cells.
Flow Cytometry Platforms
Creative Proteomics uses an advanced analysis platform to provide you with fast and reliable analysis results.
Workflow of Flow Cytometry Analysis
Advantages Our Flow Cytometry Service
- Comprehensive Capabilities: Supports cell cycle, apoptosis, immune cell typing, antigen profiling, and cell sorting.
- High Sensitivity and Precision: Advanced multi-parameter cytometers ensure accurate quantification and population analysis
- Customizable Workflows: Tailored analysis plans for diverse research goals, from basic detection to complex multi-color assays.
- High Capacity: Process hundreds of thousands of samples quickly and cost-effectively, ready to meet high research demand.
- Expert Technical Team: Experienced scientists provide guidance on panel design, gating, and data interpretation.
- High Throughput and Efficiency: Rapid processing and analysis of large sample volumes with consistent data quality.
- Detailed Reporting: Delivers clear plots, quantitative summaries, and analytical insights for research or publication.
Applications of Flow Cytometry Service
Flow cytometry is a versatile and powerful analytical technology widely used in cell biology, immunology, oncology, hematology, and clinical diagnostics. It enables rapid, quantitative, and multiparametric analysis of individual cells within heterogeneous populations. Common applications include cell cycle and apoptosis analysis, immune cell classification, surface and intracellular marker detection, and functional assays such as cytokine secretion or calcium flux measurement. In biomedical research, flow cytometry supports studies on immune regulation, cancer progression, drug response, and vaccine development. In biopharmaceutical and clinical settings, it serves as a key tool for cell quality assessment, therapeutic monitoring, and disease diagnosis, making it indispensable in both fundamental research and translational medicine.
Demo Result of Flow Cytometry Service
Flow Cytometry chart
FAQs of Flow Cytometry Service
Why Is Flow Cytometry Essential for Cellular Analysis?
Flow cytometry is an indispensable tool for modern cellular research, enabling multiparameter analysis of individual cells within heterogeneous populations. By combining light-scattering and fluorescence-based detection, flow cytometry provides quantitative information on cell size, granularity, and biomarker expression. This capability allows precise assessment of cell health, phenotype, and function in real time, which is critical for immunology, oncology, and stem cell studies. Compared to conventional microscopy or bulk assays, flow cytometry offers higher throughput, superior statistical accuracy, and single-cell resolution—making it an essential technology for quantitative biology.
What Are FSC, SSC, and Fluorescence Channels in Flow Cytometry?
In flow cytometry, FSC (Forward Scatter) measures cell size, while SSC (Side Scatter) reflects internal complexity or granularity. Together, these parameters provide structural information about cells. Fluorescence channels detect signals emitted by fluorochrome-conjugated antibodies or dyes that bind to specific cellular targets. Multi-laser systems allow simultaneous excitation of multiple fluorochromes, enabling high-dimensional phenotyping of complex cell populations.
Why Do Some Cell Populations Show Overlapping or Unclear Boundaries in Flow Cytometry Plots?
Signal overlap can arise from several factors, including fluorochrome spectral spillover, high background fluorescence, or cell heterogeneity. To mitigate this, compensation and panel optimization are critical. Compensation mathematically corrects fluorescence spillover between channels, while panel design minimizes spectral overlap through proper fluorochrome selection. Additionally, ensuring consistent instrument calibration and sample preparation greatly improves resolution between cell subsets.
Can Flow Cytometry Detect All Cell Types or Markers?
While flow cytometry is highly versatile, detection depends on antibody availability, fluorochrome brightness, and antigen expression levels. Very low-abundance markers may require signal amplification or advanced instruments with higher sensitivity. No single assay can simultaneously detect every cellular marker; therefore, panel design should be guided by clear biological questions and validated antibody performance.
Why Are Some Cells Lost During Flow Cytometry or Sorting?
Cell loss may occur due to sample aggregation, excessive shear stress, or suboptimal buffer conditions. Maintaining single-cell suspensions, using appropriate sheath fluids, and minimizing mechanical stress can reduce loss. For fragile cells, adjusting flow rates or nozzle sizes may further improve recovery. CP employs optimized protocols to ensure high cell viability and reproducibility in all sorting and analysis workflows.
Do I need to provide reagents for the Flow Cytometry analysis?
In most cases, we provide the necessary reagents for Flow Cytometry. However, if you require specific, custom, or rare reagents (e.g., specific monoclonal antibodies or probes), you may need to provide those. We can discuss the options available for your analysis.
What should I do if I have specific requirements or custom requests?
We offer custom Flow Cytometry services tailored to your specific needs. Whether it's a unique panel design, a rare marker, or specialized data analysis, our team can work with you to create a solution that fits your project.
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