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Top-Down based Sequencing Service

Top-Down based Sequencing

In top-down proteomics, intact protein molecular ions generated by ESI or MALDI are introduced into mass analyzer and are subjected to gas-phase fragmentation. Top-down analysis facilitates direct observation of C- and N-termini for identification of truncations and distinguish one isoform from the other by different sequences.

Compared with conventional Edman degradation, Creative Proteomics can provide both N- and C-terminal sequencing services, using MALDI ISD mass spectrometry under Top-Down strategy. even when the termini are blocked by modification, including N-terminal acetylation, N-terminal pyroglutamic acid, sequence truncations and signal sequences. This analysis can be also used for characterization of protein degradation, and enzymatic cleavage.

The top-down sequence analysis confirms 20-80 residues from both the N-terminal and the C-terminal of the intact protein.

Creative Proteomics also provide the following bioinformatics services in Top-Down based Sequencing:
 Functional annotation and enrichment analysis
 Clustering analysis
 Network analysis
 Statistical analysis  

Please feel free to Contact Us to discuss your projects. We hope you will find that we can meet your research needs.

* For Research Use Only. Not for use in diagnostic procedures.
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