Phospholipids Analysis Service


Phospholipids Analysis Service

Phospholipids are a type of lipids that are a major  component of cell membranes. They can form lipid bilayers for their amphiphilic  characteristic. The structure of a phospholipid molecule typically consists of  two hydrophobic fatty acid "tails" and a hydrophilic "head"  consisting of a phosphate group. These two components are joined together by a  glycerol molecule. The phosphate group can be modified with simple organic  molecules such as choline. The first phospholipid discovered in 1847 as such in  biological tissues was lecithin, or phosphatidylcholine, in the egg yolk of  chickens by the French chemist Theodore Nicolas Gobley. Biological membranes in  eukaryotes also contain another kind of lipid, sterol, interspersed among the  phospholipids and together they offer membrane fluidity and mechanical strength.

Phospholipid  synthesis happens in the cytosole adjacent to ER membrane which is studded with  proteins that act in synthesis and allocation (flippase and floppase). Ultimatelya  vesicle will bud off from the ER containing phospholipids destined for the  cytoplasmic cellular membrane on its exterior leaflet and phospholipids  destined for the exoplasmic cellular membrane on its inner leaflet.

Phospholipids  have been widely applied to prepare ethosomal, liposomal and other  nanoformulations of topical, oral and parenteral drugs for differing reasons  like improved bio-availability, increased penetration andreduced toxicity.  Liposomes often consist of phosphatidylcholine-enriched phospholipids and may contain mixed phospholipid  chains with surfactantproperties. The ethosomal formulation of  ketoconazole using phospholipids is a promising option for transdermal delivery  in fungal infections.

Currently, a reliable and  reproducible method using highly  sensitive LC-MS/MS platform for the  identification and quantification of diverse  phospholipid species in different sample types has been established by the  scientists at Creative Proteomics, which can satisfy the needs of academic and industrial  study in your lab.

Platform

Summary

Identification and quantification of diverse  phospholipids  by mixed organic solvent extraction in cells or tissue. These phospholipid  species can  be measured in a Phospholipid Panel by targeted LC-MS/MS or by shotgun  approaches.

Sample Requirement

Report

Representative phospholipid  species in Phospholipids Panel 1

Representative phospholipid  species in Phospholipids Panel 2

Ordering Procedure:

Ordering Procedure

*If your organization requires signing  of a confidentiality agreement, please contact us by email.

Staffed by experienced biological  scientists, Creative Proteomicscan provide a wide range of services ranging from the  sample preparation to the lipid extraction, characterization, identification and quantification. We promise  accurate and reliable analysis, in  shorter duration of time! You are welcome to discuss your  project with us.


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