Parallel Reaction Monitoring (PRM) Service Online Inquiry

Parallel  reaction monitoring (PRM) is  an ion monitoring technique based on high-resolution and high-precision mass  spectrometry. The principle of this technique is comparable to SRM/MRM, but it  is more convenient in assay development for absolute quantification of proteins  and peptides. It is most suitable for quantification of multiple proteins in  complex sample with an attomole-level detection.

Parallel Reaction Monitoring (PRM)

PRM is based on Q-Orbitrap as the representative quadrupole-high resolution mass spectrum  platform. Unlike the SRM, which performs one transition at a time, the PRM performs  a full scan of each transition by a precursor ion, that is, parallel monitoring  of all fragments from the precursor ion. First, the PRM uses the quadrupole  (Q1) to select the precursor ion, and the selection window is usually m/z≤2; then, the precursor  ion is fragmented in the collision cell (Q2); finally, Orbitrap replaces Q3,  scans all product ions with high resolution and high accuracy. Therefore, PRM technology not only has the SRM/MRM target quantitative analysis  capabilities, but also have the qualitative ability. (1) The mass accuracy can  reach to ppm level, which can eliminate the background interference and false  positive better than SRM / MRM, and improve the detection limit and sensitivity  in complex background effectively; (2) Full scan of product ions, without the  need to select the ion pair and optimize the fragmentation energy, easier to  establish the assay; (3) a wider linear range: increased to 5-6 orders of  magnitude.

Highlight of SRM/MRM technique:

  • High  sensitivity;
  • High  throughput;
  • Easier  to develop assay.

PRM Analysis Services at Creative Proteomics offers you a state-of-the-art strategy of  proteins analysis. The workflow is based on the following sections:

  • The PRM assay development. This is the core of the approach. First, 2 or 3 targeted peptides should be selected from a spectral library  generation or Skyline software. The selected peptides should be unique to the  protein of interest and easily detected by LC-MS. They also require no missed  cleavage sites and no frequently modified amino acids. Second, a working linear  curve was built with the corresponding concentration ratios on the x-axis and  peak area ratios on the y-axis.
  • Analysis of the sample. After  digestion, the sample is performed on the Q-Oribtrap mass spectrometer, such as  Q Exactive or Fusion mass spectrometer.
  • Data analysis. Data  will be analyzed with Skyline software.

PRM application

  • Verification  of iTRAQ differential protein;
  • Verification  of label-free differential protein follow-up;
  • Protein  and peptide absolute quantification;
  • Quantification  of disease markers, the establishment of diagnostic models;
  • Phosphorylation  protein quantification, methylation protein quantification;
  • Other  post-translationally modified protein quantification;
  • Quantitative  analysis of pathways.

How To Place Your Order?

Ordering Procedure

*If your  organization requires signing of a confidentiality agreement, please contact us  by email.

As one of the leading omics industry  company in the world! Creative Proteomics now is opening to provide proteomic  analysis of post-translational modifications service for our customers. With  over 8 years experience in the field of bioinformatics, we are willing to  provide our customer the most outstanding service! Contact us for all the  detailed information!

* For Research Use Only. Not for use in diagnostic procedures.
Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

* Email
* Phone
* Service & Products of Interest
Services Required and Project Description
* Verification Code
Verification Code