Protein-Protein Interaction Networks

Proteins are vital macromolecules that facilitate diverse biological processes at both cellular and systemic levels. Enormous molecular processes are regulated via a large number of protein components organized by Protein-Protein Interactions (PPIs), which refer to intentional physical contacts established between two or more proteins and resulted in specific biochemical events. Such interactions undertake at the core of the entire interactomics system of the living cells, unsurprisingly, specific PPIs are identified with a correlation of multiple diseases.

Protein-protein interaction hot spots and allosteric sites.

Figure 1. Protein-protein interaction hot spots and allosteric sites. (Turnbull A P. et al.; 2014)

The discovery and verification of protein-protein interaction is the first step to understand where, how and under what conditions these proteins interact in vitro / in vivo and their functional implications underlying behind interactions. As shown in Table 1, Some popular methods in PPI studies listed below.

Table 1. The most popular methods in PPI studies

Protein-Protein Interaction Analysis at Creative Proteomics

Various techniques have been applied in our group to study PPIs. Each method has its own advantages and limits. We instruct the best suitable method to our customers. Our services include but are not limited to:

Co-IP is a useful in vitro method to evaluate proteins that involved in the complex and whether it binds to each other tightly. PPIs indentification utilizes target protein-specific antibodies to capture proteins that are bound to the specific target protein. Co-IP enables to capture and purify not merely the primary target, but as well other macromolecules that are involved in the interactions.

The pull-down assay is capable of detecting a physical interaction between two or more proteins and identifies previously unknown PPIs. In a pull-down assay, a bait protein is tagged and immobilized to affinity resin. When a sample incubates with the bait proteins, proteins binding to the bait protein will be captured and “pulled down”.

Crosslinking protein interaction analysis is suitable for transient or weak interaction, which can be performed in vivo or in vitro. In this method, analytical solution, like crosslinking reagents or crosslinkers, enable to arrest protein-protein complexes by covalently binding, correspondingly followed with consequent isolation and characterization.

Label transfer has been applied for detecting transient or weak PPIs that are difficult to capture using other in vitro detection strategies. A label transfer reagent will be performed to tag proteins that interact with a protein of interest. The development of new non-isotopic reagents and methods enhanced a simpler and more accessible label transfer analysis.

Far-western blot, based immunoblotting procedure, also detects PPIs in vitro and do not require to preserve the native state of target protein. In this method, a purified and labeled "bait" protein is used to probe the target "prey" protein on the membrane.

The result of two or more proteins that interact with a specific functional objective is available to be demonstrated in several different ways. The measurable effects of protein interactions have been outlined as follows:

Creative Proteomics has a team of scientists with specific experience in protein-protein interaction studies, our protein-protein interaction network platform will assist you to decipher protein-protein interaction and expand the outlook on your research.

Our ordering procedure is as follows. If you have any questions or specific requirements, please feel free to contact us.

Ordering Procedure


1. Turnbull A P, Boyd S M, Walse B. Fragment-based drug discovery and protein–protein interactions. Research and Reports in Biochemistry, 2014, 4: 13-26.

* For Research Use Only. Not for use in diagnostic procedures.
Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry
Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

  • Verification Code
Copyright © 2021 Creative Proteomics. All rights reserved.