If an exact knowledge of protein/peptide quantities is required for further applications, quantitative amino acid analysis, qAAA, is a suitable assay, which can not only determine protein quantities precisely, but also provide detailed information regarding the relative amino acid composition and free amino acids. The AAA procedure includes hydrolysis, separation, detection and quantification.
Hydrolysis is typically achieved under acid conditions. A standard procedure is hydrolysis with 6 M hydrochloric acid (24 hours, 110°C). Fragile amino acids, especially tryptophan and cysteine, will be partially destroyed. And then hydrolyzed samples (amino acids) are derivatized pre-column or post column for sensitive detection, separated by RP/SCX column. The use of internal and external standards of known amount is crucial for accurate quantification of each amino acid.
Protein characterization, concentration, content, molar ratio, and extinction coefficients can all be determined by using our amino acid analysis services.
Our Amino Acid Analysis Service
Creative Proteomics offers comprehensive amino acid analysis and amino acid quantification services on biological compounds, foods, tissue, biological fluids, and drug samples. Using UV spectrophotometry, Creative Proteomics can obtain accurate data on proteins, peptides, and amino acids of a particular sample.
Our amino acid analysis service consists of:
*Quantification of protein or peptide
*Quantification of protein traces
*Accurate quantification of protein or peptide
Sample Format: Solid samples or in solution
Dry protein/peptide samples: 100 µg of sample or greater at a concentration of approximately 0.5-2 mg/ml.
liquid material: >100 microliters at a minimum concentration of 0.1 micrograms per microliter.
Please consult us for other sample requirements
Equipped with automatic amino acid analyzer, Creative Proteomics can provide highly reproducible quantitative analysis of protein/peptide samples the customers submit, for estimation of amino acid composition of specific proteins, and purity determination.