Protocol

Proteomics Metabolomics Lipidomics Glycomics

Protocols for Preparation of Cellular and Subcellular Extracts

Protocols for Extraction and Solubilization of Proteins for Proteomic Studies

Protocols for Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis

Protocols for Preparation of Bacterial Samples for 2-D PAGE

Protocols for Preparation of Yeast Samples for 2-D PAGE

Protocols for Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis

Protocols for Preparation of Plant Protein Samples for 2-D PAGE

Protocols for Prefractionation of Complex Protein Mixture for 2-D PAGE Using Reversed-Phase Liquid Chromatography

Protocols for Protein Identification by In-Gel Digestion and Mass Spectrometric Analysis

Protocols for Peptide Sequences of 2-D Gel-Separated Protein Spots by Nanoelectrospray Tandem Mass Spectrometry

Protocols for Identification of Proteins by MALDI-TOF MS

Protocols for Analysis of the Proteomes in Human Tissues by In-Gel Isoelectric Focusing and Mass Spectrometry

Protocols for Liquid Chromatography Coupled to MS for Proteome Analysis

Protocols for Quantitative Analysis of Proteomes and Subproteomes by Isotope-Coded Affinity Tags and Solid-Phase Glycoprotein Capture

Protocols for Identification of Posttranslational Modification by Mass Spectrometry

Protocols for Identification of Protein Phosphorylation Sites by Mass Spectrometry

Protocols for The Yeast Two-Hybrid System for Detecting Interacting Proteins

Protocols for A High-Resolution Two Dimensional Gel- and Pro-Q DPS-Based Proteomics Workflow for Phosphoprotein Identification and Quantitative Profiling

Protocols for Enrichment and Characterization of Phosphopeptides by Immobilized Metal Affinity Chromatography (IMAC) and Mass Spectrometry

Protocols for The Use of Titanium Dioxide Micro-Columns to Selectively Isolate Phosphopeptides from Proteolytic Digests

SILAC Protocol for Global Phosphoproteomics Analysis

Protocol for Reverse-Phase Diagonal Chromatography for Phosphoproteome Research

Enzyme-Linked Immunosorbent Assay (ELISA) Protocol

Protocol for Differential Detergent Fractionation of Eukaryotic Cells

Protocol for 2-D PAGE of High-Molecular-Mass Proteins

Protocol for Silver Staining of 2-D Gels

Protocol for Zn²⁺ Reverse Staining Technique

Protocol for Lowry Protein Assay

Protocol for Cellulose Acetate Electrophoresis (CAE)

Protocol for Polyacrylamide Gel Electrophoresis (PAGE)

Protocol for Capillary Electrophoresis

Protocol for Sandwich ELISA

Protocol for Competitive ELISA

Protocol for Direct ELISA

Protocol for Indirect ELISA

Protocol for Biuret Protein Assay

Protocol for Bradford Protein Assay

Protocol for Bicinchoninic Acid (BCA) Protein Assay