Extracellular vesicles (EVs) occur in most bodily fluids and cell culture supernatants. Exosomes, a part of two EV subgroups, are small vesicles (traditionally considered 50-150 nm) originating from an intracellular compartment, called Multivesicular Bodies (MVB, or Late Endosomes) and released out of the cell. Exosomes are involved in cell-to-cell communication through the biological transfer of lipids, proteins, DNAs, RNAs, mRNAs, and miRNAs.
Exosomes have various composition, including proteins, nucleic acids, lipids, and other metabolites. Lipids play essential roles not only in the structures of exosomal membranes, but also in exosome formation and releasing to the extracellular environment. Knowing the exosome lipid composition is important to understand the biology of vesicles and to investigate possible medical applications. Exosome lipidomics analysis can help us to understand vesicle biogenesis and function, identify lipid-based biomarkers and unravel its monitoring function in diseases.
Figure 1. Lipid composition of exosomes. (PC: phosphatidylcholine; PE: phosphatidylethanolamine; SM: sphingomyelin; CER: ceramide; TG: triacylglyceride; DG: diacylglyceride; CE: cholesteryl ester; S1P: sphingosine-1-phosphate)
Creative Proteomics provides exosome lipidomics analysis to help you identify and quantify the lipids in the exosomes quickly, accurately and efficiently. Sophisticated shotgun method and targeted lipidomic assays will be used for in-depth analysis of the exosome lipidomics. We can use powerful facilities to analyze the complexity of lipids, such as Mass spectrometry (MS), high-performance liquid chromatography (HPLC), liquid chromatography coupled MS (LC-MS), nuclear magnetic resonance spectroscopy (NMR), etc.
With cutting-edge facilities and optimized protocols, we can provide comprehensive services for exosome research, including:
We can accept various types of samples, including but not limited:
|Serum||500 ul-1 ml|
|Plasma||500 ul-1 ml|
|Ascites Fluid||500 ul-1 ml|
|Spinal Fluid||5 ml-10 ml|
|Urine||5 ml-10 ml|
|Cell Media||5 ml-10 ml|
|If you want to know specific samples requirements, please feel free to contact us.|
At Creative Proteomics, we will provide a detailed report to our clients. Our reports contain:
Exosome lipidomics service at Creative Proteomics helps our clients obtain the most information from your exosomes to accelerate basic exosome research, circulating biomarker discovery, or other exosome-related researches. In addition, we have other advanced platforms to isolate, purify, and study exosomes. As every project has different requirements, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you.