Peptide purity is the amount of correct peptide relative to all analytes. The peptide purity is determined by HPLC analysis, often monitored at 214 nm where the peptide bond absorbs. The purity of peptide is a critical factor that affects the results of a research project, which leads to peptide purity analysis widely performed in various industries. Common contaminants of peptide product are resulted from chemical synthesis, culturing medium, or incorporated from extraction and purification procedures. The impurities consist of peptides with deletion sequences, truncated sequences, incomplete deprotection, as well as byproducts generated during synthesis or the final cleavage.
Recommended Peptide Purity for Various Applications
|Peptide Purity Range||Applications|
Suitable for quantitative assays and structural studies
|>85%||Suitable for most qualitative and semi-quantitative biochemical assays|
|>70%||Suitable for a number of screening and research applications|
Overview of Peptide Purity Analysis Service
Creative Proteomics has already developed a highly sensitive and professional platform. We can provide peptide purity analysis with the help of analytical reversed phase HPLC in combination with mass spectrometry.
The chromatogram produced by reversed phase HPLC can be used for the determination of the peptide purity. The peptide purity is quantified by the peak area of the final purified peptide corresponds to the area of the main peak in relation to the total area of all peaks detected within the chromatogram. And we can get the number and relative amounts of possible by-products through calculating the peak areas.
In addition to RP HPLC, we can provide the information about the molecular mass of a peptide determined by mass spectrometry. If the samples contain impurities in the peptide products, the mass spectrometry can show them. There are two mass spectrometers routinely used, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and electrospray ionization mass spectrometry (ESI MS).
In addition, we can provide amino acid analysis (AAA) to determine the amino acid composition in a peptide or protein product, which ensures experimental accuracy and consistency.
At Creative Proteomics, many excellent and experienced experts will optimize the experimental protocol according to your requirement and guarantee the high-quality results of peptide purity analysis. As every project has different requirements, please contact our specialists to discuss your specific needs.