Lipidomics is a discipline that analyzes and identifies lipid metabolites and their interacting molecules in organisms, tissues or cells to understand the structure and function of lipids, and then reveals the relationship between lipid metabolism and the physiological and pathological processes of cells, organs and even organisms. With the advancement of mass spectrometry, lipidomics has shown a wide range of applications in the identification of lipid biomarkers, diagnosis of diseases, discovery of drug targets and lead compounds, and study of drug mechanisms of action.
Targeted lipidomics is based on high-resolution mass spectrometry and isotope internal standard, and uses PRM (parallel reaction monitoring) targeted analysis technology to specifically acquire the signals of multiple lipid molecules (such as dozens of target lipid molecules) simultaneously to obtain their absolute contents and meet the needs of targeted detection and validation of target lipids.
Lipids are hydrophobic or amphipathic small molecules which include fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides and phospholipids. The crucial role of lipids in biological physiology is evident not only in energy storage and structural components of cellular membranes, but also in signal transduction, membrane trafficking and morphogenesis. It is known that mammalian cells contain 1000-2000 types of lipids, but there are many more than that, and it is conservatively estimated that the major lipid species theoretically contain nearly 200000 lipids. Lipids can be broadly classified into three categories, namely, polar lipids including glycerophospholipids and sphingolipids, and nonpolar lipids including glycerol esters and other lipid metabolites such as fatty acids, which are the precursors of biologically active lipid molecules.
The National Institutes of Health (NIH) classifies lipids into eight specific categories including fattyacids, sphingolipids, glycerolipids, glycerophosph1ipids, sterol lipids, saecharolipids, pregnenolipids, and polyketides.
Lipidomics Analysis Workflow
Equipped with advanced tandem mass spectrometry for the determination of lipid composition, powerful bioinformatic analytic systems, and a panel of experienced scientists and technicians, Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.
With an integrated set of separation, characterization, identification and quantification system, featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective targeted lipidomics services as below.
Featured Lipid Analysis Service:
Fatty Acids Analysis Service
Fatty Acids Derivatives Analysis Service
Fatty Acids Metabolism Analysis Service
Glycerolipids Analysis Service
Glycerophospholipids Analysis Service
Other Analysis Services
|Standard analysis||Quality control||Get high quality data|
|Method evaluation||Assess the quality of the established method|
|Difference analysis (histogram, violin plot, etc.)||Assess group differences for each lipid molecules|
|Advanced analysis||Cluster Analysis||Exploring the content trend pattern of lipid molecules|
|Machine learning||Biomarkers screening with good diagnostic performance|
- Platform advantages: orbitrap mass analyzer, ultra-high resolution mass spectrometry, high-quality data
- Wide applicability: no species restriction, no standard product restriction
- High throughput: detect dozens of lipid molecules at one time, saving samples and costs
- Strong quantitative ability: the sensitivity can reach ppm level, and the linear range can reach 5-6 orders of magnitude
- State of art facilities
- Constantly optimized protocol and analytical software
- Professional experiment design
- Quick turnaround time