2-Bromopalmitate LC-MS Detection Service for Fast, Reliable Results

2-Bromopalmitate (2-BP) is a potent inhibitor of palmitoylation, specifically targeting palmitoyl acyl transferases (PATs). By irreversibly blocking the DHHC enzyme family, 2-BP effectively disrupts palmitoylation processes for proteins like MyrGCK(NBD) and FarnCNRas(NBD).

Creative Proteomics leverages the SCIEX Triple Quad™ 5500 plus mass spectrometry system, offering a highly sensitive, specific, stable, and rapid analytical method tailored to accurately quantify 2-BP levels, benefiting pharmaceutical research and drug developers.

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Why Use LC-MS
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What is 2-Bromopalmitate (2-BP) and Why Does It Matter?

2-BP is a synthetic analog of palmitic acid widely known for its role as an irreversible inhibitor of protein palmitoylation. Palmitoylation—a lipid modification that attaches fatty acids to proteins—is crucial for regulating protein localization, stability, and signaling.

Researchers frequently use 2-BP to:

Investigate lipid signaling pathways
Understand protein transport and localization
Explore disease mechanisms in metabolic disorders, cancer, and neurological conditions

This compound acts by inhibiting the enzyme palmitoyl acyltransferase (PAT), which transfers palmitate to protein substrates. Blocking this process allows scientists to study protein function more precisely, especially in dynamic environments like cancer progression or neurodegenerative disease pathways.

Its powerful inhibitory effect makes 2-BP an invaluable tool in proteomic and metabolomic studies, especially when used alongside advanced analytical tools like LC-MS.

Why Use LC-MS for 2-BP Detection? Benefits Over Other Methods

Compared to other analytical techniques like GC-MS or NMR, LC-MS stands out for:

Superior Sensitivity: Detection limits as low as 1.67 ng/mL.

Higher Specificity: Reduced false positives through precise ion filtering.
Wide Dynamic Range: Suitable for both trace-level and bulk quantification.
Fast Throughput: Automation-ready and ideal for high-volume projects.

It also handles a variety of sample types—from tissue extracts to urine and plasma—without requiring derivatization. This versatility simplifies workflows and increases speed.

Creative Proteomics integrates targeted metabolomics and untargeted metabolomics approaches to offer tailored 2-BP profiling for both exploratory and confirmatory research.

We utilize cutting-edge tools like the SCIEX Triple Quad™ 5500+ system, ensuring every molecule of 2-BP is accounted for. Calibration curves further enhance the method's reliability, helping quantify 2-BP even at low concentrations.

Our service also complements our Palmitoylation Site Identification Service, helping researchers understand where and how lipid modification occurs in specific proteins.

Applications of 2-BP LC-MS Detection

2-BP detection is widely adopted in:

Pharmaceutical Development: Screening inhibitors targeting lipid-modified proteins
Biotechnology Research: Exploring post-translational modifications
Clinical Studies: Investigating lipid-related disease biomarkers

Our Workflow: How Creative Proteomics Detects 2-BP

Figure 1: High-resolution mass spectrometry systems in our LC-MS lab.

Step-by-Step Service Workflow:

Sample Submission: Accepts serum, plasma, urine, cell lysates, and tissue.
Sample Preparation: Internal standards added; matrix interference removed.
LC-MS Analysis: Conducted using validated UHPLC and triple quadrupole MS systems.
Data Processing: Generation of EIC and standard curves.
Comprehensive Reporting: Includes QC metrics and statistical data.

We maintain end-to-end traceability and offer direct communication with our technical specialists throughout the project.

Quality Control and Validation: Our Gold Standard

We adhere to strict international validation protocols to ensure reliability:

Sensitivity: Limits of detection down to 1.67 ng/mL
Precision: Coefficient of Variation (CV%) typically<10%
Repeatability & Trueness: Consistent quant results across replicates
Stability Testing: Ensures compound stability during sample storage and processing
Internal QA Practices:
Daily calibration of equipment
Inclusion of multiple QC replicates in every batch

Our Metabolomics Technology Advantages:

Comprehensive Metabolite Database:

Access to over 500 metabolites, including a proprietary database of 450+ verified standards, each featuring detailed MS1, MS2, or retention time data for precise identification.

Broad Metabolic Pathway Coverage:

Thorough profiling of metabolites across major metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway.

Versatile Sample Compatibility:

Capable of analysing diverse sample types such as cells, tissues, blood, feces, urine, nematodes, and bacterial cultures.

Robust Quality Controls:

Utilization of isotope-labelled internal standards and quality control (QC) samples with randomized run sequences ensures consistently accurate data.

Highly Reproducible Workflows:

Standardized processes designed specifically to ensure reliable outcomes even when handling large sample volumes.

Expert Consultation and Data Validation:

Dedicated technical specialists available for data interpretation, consultation, and rigorous data validation to ensure optimal research quality.

Platform

Sample Requipment

Sample Volume Guidelines

Sample Category	Sample Type	Recommended Amount (/sample)	Minimum Amount (/sample)
Cell Samples	Cells	5×10⁷ cells	1×10⁷cells
Biofluid Samples	Serum	100 µL	> 100 µL
	Plasma	100 µL	> 100 µL
	Urine	500 µL	> 200 µL
Animal Samples	Tissue	100 mg	100-200 mg
	Feces	100 mg	25 mg

Recommended Biological Replicates:

Cell samples: Minimum of 5 biological replicates per group.
Animal models: Minimum of 6 biological replicates per group.
Clinical samples: Minimum of 30 biological replicates per group.

Demo

Below is an illustrative example of typical metabolomics results:

Extracted Ion Chromatogram (EIC) and Standard Curve

FAQs: What Clients Ask About 2-BP LC-MS Detection

What types of samples can you analyze for 2-BP?

We accept serum, plasma, urine, tissue, and cultured cells.

What's the detection limit for 2-BP?

As low as 1.67 ng/mL using LC-MS/MS.

Do you offer batch pricing for large studies?

Yes. Contact us for a tailored quote based on sample count.

How are results delivered?

Via secure, downloadable report containing raw data, processed results, and QC validation.

Can LC-MS be combined with other services?

Absolutely. Many clients integrate 2-BP detection with untargeted metabolomics or PTM analysis for deeper insight.

Learn about other Q&A about proteomics technology.

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Featured Publications Using Creative Proteomics' LC-MS Services

Calcium homeostasis and stable fatty acid composition underpin heatwave tolerance of the keystone polychaete Hediste diversicolor. Environmental Research. 2021. https://doi.org/10.1016/j.envres.2021.110885
MS-CETSA functional proteomics uncovers new DNA-repair programs leading to Gemcitabine resistance. Research Square. 2024. https://doi.org/10.21203/rs.3.rs-4820265/v1
White matter lipid alterations during aging in the rhesus monkey brain. GeroScience. 2024. https://doi.org/10.1007/s11357-024-01353-3
Pan-lysyl oxidase inhibition disrupts fibroinflammatory tumor stroma, rendering cholangiocarcinoma susceptible to chemotherapy. Hepatology Communications. 2024. https://doi.org/10.1097/HC9.0000000000000502

More Publications

References

Jia Z, Qiu Q, He R, Zhou T, Chen L. Identification of Metabolite Interference Is Necessary for Accurate LC-MS Targeted Metabolomics Analysis. Anal Chem. 2023;95(20):7985-7992. doi:10.1021/acs.analchem.3c00804
Chen L, Lu W, Wang L, et al. Metabolite discovery through global annotation of untargeted metabolomics data. Nat Methods. 2021;18(11):1377-1385. doi:10.1038/s41592-021-01303-3
Jang C, Chen L, Rabinowitz JD. Metabolomics and Isotope Tracing. Cell. 2018;173(4):822-837. doi:10.1016/j.cell.2018.03.055
Li F, Song J, Zhang Y, et al. LINT-Web: A Web-Based Lipidomic Data Mining Tool Using Intra-Omic Integrative Correlation Strategy. Small Methods. 2021;5(9):e2100206. doi:10.1002/smtd.2021

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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