Lipidomic profiling is the systematic analysis and identification of lipids in organisms, tissues or cells and the molecules with which they interact. In addition, the structure and function of lipids can also be understood, so as to reveal the relationship between lipid metabolism and the physiological and pathological processes of cells, organs and even the body. The contents of lipidomics research mainly include analysis and identification of lipids and their metabolites, lipid function and metabolic regulation, lipid metabolism pathways and networks.
At Creative Proteomics, we can provide a wide range of lipidomics profiling.
|Lipid Function and Metabolic Regulation Analysis||The study of lipid function and metabolic regulation mainly uses large-scale analysis methods of lipids and their metabolites, combined with genomics and proteomics technology, and can be used to analyze the composition and dynamic changes of key protein complexes related to lipid and its function and regulation under various conditions in various basic cell and animal models. In order to better understand the function and metabolic regulation of the corresponding lipid.|
|Lipid Metabolism Pathway and Network Analysis||The solution can be used to elucidate the physiological or pathological mechanism of corresponding lipid metabolism regulation, reveal key regulatory sites and their corresponding key genes/proteins/enzymes. It can also be used to excavate new drug target systems and provide data foundation for the prevention, diagnosis and treatment of related diseases and the development of new drugs.|
|Untargeted Lipidomics||Untargeted lipidomics, based on mass spectrometry, can analyze hundreds and thousands of individual lipid species for unbiased qualitative and quantitative analysis of a lipidome.|
|Targeted Lipidomics||Targeted lipidomics involves the identification and quantification of defined groups of known lipids across large sample sets. We can provide separation, characterization, identification and quantification for various types of lipids, including fatty acids, fatty acids derivatives, glycerolipids, glycerophosholipids, sphingolipids, sterols, and so on.|
|Exosome Lipidomics||Exosomes are small vesicles released from cells after fusion of the multivesicular bodies (MVBs) with the plasma membrane, which is reported to have the highest lipid-to-protein ratio of all classes of extracellular vesicles. Exosome lipidomics is a useful tool for lipid-based biomarkers and studies of vesicle biogenesis and function.|
|MALDI Imaging Lipidomics Service||MALDI imaging mass spectrometry (MALDI-IMS), in which the ion source is matrix-assisted laser desorption ionization (MALDI) and a thin tissue section is moved in two dimensions while the mass spectrum is recorded, is useful to study the distribution of the lipids in tissues. Creative Proteomics provides a well-established solution of MALDI-Imaging.|
Figure 1. Analytical Workflows in Lipidomics (Hyötyläinen et al.,2015)
The techniques of lipidomics research mainly include the extraction, separation, analysis and identification of lipids and the corresponding bioinformatics techniques. CPR mainly provides lipidomic analysis services based on biological mass spectrometry technology. The lipid analysis strategy based on mass spectrometry mainly includes liquid chromatography-mass spectrometry and shotgun lipidomics.
Our experts have many years of experience in lipidomics, bioinformatics, and various application fields from food, agriculture, disease research to pharmacy, can provide you with detailed plans, implementation and provide you with corresponding experiments report. Whether you need a solution for lipidomics or conducting targeted or non-targeted lipid research, we will work closely with you to determine the research purpose and develop a customized plan. If you have any questions or specific requirements, please feel free to contact us.
1. Hyötyläinen T, Orešič M. Analytical lipidomics in metabolic and clinical research. Trends in Endocrinology & Metabolism, 2015, 26(12): 671-673.
2. Li Q, Liang X, Xue X, et al. Lipidomics Provides Novel Insights into Understanding the Bee Pollen Lipids Transepithelial Transport and Metabolism in Human Intestinal Cells. Journal of Agricultural and Food Chemistry, 2019, 68(3): 907-917.