A steroid is an organic compound with four rings arranged in a specific configuration, such as dietary lipid cholesterol, estradiol, testosterone and dexamethasone. Steroids function as important components of cell membranes and many of them are signaling molecules. The core structure of the steroid is composed of seventeen carbon atoms, bonded in four "fused" rings: three six-member cyclohexane rings and one five-member cyclopentane ring. Hundreds of steroids are found in plants, animals and fungi. All steroids are manufactured in cells from the sterols lanosterol (animals and fungi) or cycloartenol (plants). Lanosterol and cycloartenol are derived from the cyclization of the triterpene squalene.
Equipped with advanced LC/MS/MS sophisticated detection techniques, and a panel of experienced scientists and technicians. Steroids analysis service is able to quantification up to 38 steroids in biological matrices such as plasma, serum, tissue, cells, and cell culture media.
Sample preparation and extraction of steroids is by either liquid-liquid extraction (LLE) or supported liquid extraction (SLE) followed by an on-line 2D polishing extraction. Target steroid analytes are chromatographically separated on a reverse-phase (RP) column. All analytes and Internal Standards are measured by ESI ionization--pos and/or neg polarity (analyte dependent). Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.
ESI Liquid Chromatography Mass Spectrometry (ESI-LC/MS)
Plasma, serum, tissue, cells, and cell culture media
Normal Volume: 500 uL
Minimal Volume: 50uL
Steroids could monitored in This Service
|∆4 Steroids could monitored in This Service|
|∆5 Steroids could monitored in This Service|
|5α-Reduced could monitored in This Service|
|Conjugated Steroids Sulfates could monitored in This Service|
|Pregnenolone sulfate||17-hydroxypregnenolone sulfate||Dehydroepiandrostenedione-sulfate|
|Androsterone sulfate||Etiocholane sulfate||Androst-5-ene-diol sulfate|
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.