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Steroids Analysis Service

A steroid is an organic compound with four rings arranged in a specific configuration, such as dietary lipid cholesterol, estradiol, testosterone and dexamethasone. Steroids function as important components of cell membranes and many of them are signaling molecules. The core structure of the steroid is composed of seventeen carbon atoms, bonded in four "fused" rings: three six-member cyclohexane rings and one five-member cyclopentane ring. Hundreds of steroids are found in plants, animals and fungi. All steroids are manufactured in cells from the sterols lanosterol (animals and fungi) or cycloartenol (plants). Lanosterol and cycloartenol are derived from the cyclization of the triterpene squalene.

Steroids Analysis Service

Equipped with advanced LC/MS/MS sophisticated detection techniques, and a panel of experienced scientists and technicians. Steroids analysis service is able to quantification up to 38 steroids in biological matrices such as plasma, serum, tissue, cells, and cell culture media.

Sample preparation and extraction of steroids is by either liquid-liquid extraction (LLE) or supported liquid extraction (SLE) followed by an on-line 2D polishing extraction. Target steroid analytes are chromatographically separated on a reverse-phase (RP) column. All analytes and Internal Standards are measured by ESI ionization--pos and/or neg polarity (analyte dependent). Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.

Technology Platform:

ESI Liquid Chromatography Mass Spectrometry (ESI-LC/MS)

Sample Type:

Plasma, serum, tissue, cells, and cell culture media

Sample Volume:

Normal Volume: 500 uL

Minimal Volume: 50uL

Steroids could monitored in This Service

∆4 Steroids could monitored in This Service
∆5 Steroids could monitored in This Service
5α-pregnane-3α,17α-diol-20-one (pdiol)Dehydroepiandrosterone5-Androstenediol
5α-Reduced could monitored in This Service
Conjugated Steroids Sulfates could monitored in This Service
Pregnenolone sulfate17-hydroxypregnenolone sulfateDehydroepiandrostenedione-sulfate
Androsterone sulfateEtiocholane sulfateAndrost-5-ene-diol sulfate

Ordering Procedure:

Ordering Procedure

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.

* For Research Use Only. Not for use in diagnostic procedures.
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