Cholesterol esters are a dietary lipid, which derived from cholesterol. Cholesterol esters are formed when the body produces enzymes to produce a metabolic reaction with the cholesterol itself. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol. Cholesterol esters are largely spread in body fluids of animals (plasma lipoproteins) and may be also found in vessel walls as fatty streaks in atherosclerosis. Cholesterol esters have a lower solubility in water due to their increased hydrophobicity.
For the analysis of cholesterol esters, we use a MS protocol that yields profiling data on all individual cholesterol ester molecular species based on the associated fatty acids. We recommend cholesterol ester profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. On special request, we can also quantitate the molecular species based on existing standards.
- ESI Liquid Chromatography Mass Spectrometry (ESI- LC/MS)
- Cultured Cells
- Cultured or uncultured primary mouse cells or cell lines
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With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.