Eicosanoids Analysis Service


Eicosanoids Analysis Service

Eicosanoids include prostaglandins (PG), thromboxanes  (TX), leukotrienes (LT), and lipoxins (LX). Prostanoids refer to PGs and TXs.  The names of prostanoids are given according to the number of carbon-carbon  double bonds in the molecule. Because of the presence of two carbon-carbon  double bonds, most of the biologically active prostaglandins and thromboxanes  are series 2 molecules. The eicosanoids with four carbon-carbon double bonds  are series 4 molecules. Prostaglandins are first shown being produced in the  prostate gland. Thromboxanes are first shown being synthesized in platelets  (thrombocytes). And leukotrienes are first identified from leukocytes.  Therefore, they were given their respective names. The lipoxins are  inflammation involved with eicosanoids, which are produced through lipoxygenase interactions.  In response to ingestion of aspirin, the synthesis of lipoxins can be increased  according. Therefore, lipoxins are powerful inflammation modulating eicosanoid  compounds. Other eicosanoid derivatives include resolvins (Rv) and the  protectins (PD).

Eicosanoids play an important role in  the inflammatory responses of the joints, skin and eyes, on the induction of  labor and on the intensity and duration of pain and fever. They also assist in  the inhibition gastric acid secretion, regulation of blood pressure through  vasodilation or constriction, and inhibition or activation of platelet  aggregation and thrombosis. The eicosanoids of greatest biological importance  to humans are a large number of molecules arachidonic acid derivatives in the  reaction pathway from linoleic acid to arachidonic acid. There is also a small  group of eicosanoids comes from eicosapentaenoic acid derived from α-linolenic  acid. The main source of arachidonic acid is cellular stores release. Inside  the cell, arachidonic acid localized primarily at the C–2 positions of membrane  phospholipids and the release is triggered by the activation of PLA2.

Eicosanoids can be synthesized in all  mammalian cells except erythrocytes. These molecules are rather powerful,  minimum amount of eicosanoids can exert potent physiological effects. At the  site of synthesis, eicosanoids exert their roles locally through  receptor-mediated G-protein linked signaling pathways. There are two main  pathways are associated with the biosynthesis of eicosanoids. Prostaglandins and  thromboxanes are generated in the cyclic pathway, while the leukotrienes are  produced in the linear pathway.

Since eicosanoids play a crucial role  in large number of disorders like asthma, cardiovascular disease, cancer and  chronic obstructive pulmonary disease, the detection and quantification of  these compounds are of great interest. Since the endogenous eicosanoids are of  rather low concentration, sensitive and specific analytical methods are  required for the reliable quantification of these compounds. HPLC-MS/MS has  emerged as one of the main techniques used for eicosanoid quantification.  Creative Proteomics has established sensitive, reliable, and accurate LC-MS/MS  method for quantification of eicosanoids.

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Eicosanoids Quantified in  This Service

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With integrated set of separation, characterization,  identification and quantification systems featured with excellent  robustness & reproducibility, high and ultra-sensitivity,  Creative Proteomics provides reliable, rapid and cost-effective eicosanoids targeted lipidomics services.


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