Ceramides Analysis Service

Ceramides Analysis Service

Ceramides are a group of lipids made up of  a fatty acid and sphingosine. Ceramides are component of sphingomyelin  lipids, which is one component of lipid bilayer. Therefore, ceramides are  highly abundant in cell membranes. In the past, it is assumed that the sole  role of ceramides in the cell membrane is acting as the supporting structural  elements. However, this assumption turns out to be false. It is shown that  ceramides takes part in a series of physiological process in the body.

Ceramides are synthesized by de novo synthesis from serine  and palmitoyl-CoA. Acting as precursors for many sphingolipids, ceramides play  a crucial role in cell differentiation, cell signaling, proliferation and  apoptosis. Catalyzed by the neutral Mg2+-dependent sphingomyelynase,  spingomyelin in cell membranes hydrolyze and form ceramides. This is the major  synthesis pathway of ceramides. Other minor pathways contributing to ceramide  homeostasis include acylation of sphingosine with distinct fatty acyl-CoAs  catalyzed by ceramidase and the hydrolysis of ceramide metabolites such as  galactosylceramide and glycosylceramide. 

Up till now, it’s still unclear how the structure of  individual ceramide species is related to their physiological functions.  However, it is shown in some reports that specific fatty acids produced in  response to some stimuli are components of ceramides. This might gives a clue  to the relationship between the structure and function for different ceramide  species. It is known that C18 ceramide suppress cell growth, while C16 and C24  ceramide species are associated with cell death. In one in vitro study, it is  indicated that by inhibiting Akt, a serine protein kinase involved in insulin  action, ceramides suppress glucose uptake. Besides, in insulin resistant animal  models, the concentration of ceramides increases in muscle, liver and adipose  tissue. The elevation of total ceramide concentrations in human muscle is  associated with peripheral insulin resistance. Recently, in obese subjects with  type 2 diabetes, it is demonstrated that increased plasma ceramides are related  to reduced insulin sensitivity.

Since intracellular involvement of ceramides and their  metabolites play a crucial role in lipotoxicity and insulin resistance, it is  important to identify the functions of specific ceramides in physiological and  pathological process. An accurate, precise and sensitive quantification method  for ceramides in plasma and tissue biopsy samples is a necessary for the  investigation of the physiological function of specific ceramides.  Diacylglycerol (DAG) kinase assay, thin-layer chromatography (TLC) detection, GC-MS  and HPLC are the quantification platforms for ceramides. However, these  platforms are not only cumbersome and time consuming, it is also difficult for  these platforms to simultaneously quantify different specific ceramide  species. Creative Proteomics has  established sensitive, reliable, and accurate GC-MS method for quantification  of ceramides and its metabolites.



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Ceramides And Their Metabolites Quantified in This Service

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Ceramides Analysis Service

With integrated  set of separation, characterization, identification and quantification systems  featured with excellent robustness & reproducibility, high and  ultra-sensitivity, Creative Proteomics provides reliable, rapid and  cost-effective ceramides targeted lipidomics services.

* For Research Use Only. Not for use in diagnostic procedures.
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