Disulfide Bond Analysis Service


Disulfide bond is a type of post-translational modifications in proteins formed between the sulfur atoms of two cysteine residues during the biosynthesis of the proteins in the cell. Disulfide bonds are important in protein folding, they play a significant role in both protein structure and function. Therefore, the analysis of disulfide bond in proteins is of great significance to reveal the higher structure and biological functions of proteins. In addition, incorrect disulfide bond formation or exchange can cause antibody aggregation, so understanding the disulfide bonding is critical for protein characterization in the biopharmaceutical production process.

Disulfide Bond Analysis

Figure 1. disulfide bonds in mature proteins

Overview of Disulfide Bond Analysis

Creative Proteomics provides services to map the disulfide bond sites of unknown proteins and verify the disulfide bonds and folding structure of protein samples. Basic steps include peptide extraction, peptide separation and data analysis.

Sample Requirement

A variety of biological samples are available for testing, such as cell, animal tissue, blood, serum and so on.

TypesVolume
Protein100ug
Cell2×107 cells
Animal Tissue1g
Plant Tissue200mg
Blood (EDTA added)1ml
Serum0.2-0.5ml
Urine2ml
MicrobesDry weighed: 200mg
If you want to know specific sample requirements, please feel free to contact us.

Advantages

Applications of disulfide bond analysis service:

Technology platform

We are specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, and we have optimized sample processing methods and a series of advanced analytical methods that provide a strong guarantee for the success of the research. As every project has different requirements, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you.

Reference
1. Bošnjak I, Bojović V, Šegvić-Bubić T, et al. Occurrence of protein disulfide bonds in different domains of life: a comparison of proteins from the Protein Data Bank[J]. Protein engineering, design & selection, 2014, 27(3): 65-72.


* For Research Use Only. Not for use in diagnostic procedures.
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  • Tel: 1-631-275-3058 (USA)
  • Tel: 44-208-144-6005 (Europe)
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