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Characterization of Protein SUMOylation


Sumoylation is a post-translational  modification of proteins by covalently attaching a small ubiquitin-related  modifier (SUMO) to the proteins. However, unlike ubiquitination, Sumoylation  does not target proteins for proteolytic degradation, it regulates protein functions  in various cellular precesses, such as, nuclear-cytosolic transport,  transcriptional regulation, apoptosis, protein stability, response to stress,  and progression through the cell cycle.

Structure  of SUMO

Although SUMO has very little sequence identity  with ubiquitin at the amino acid sequence, it has a nearly identical structural  fold. There are four SUMO paralogues. Usually, SUMO has around 100 amino acids  in length. The exact length and mass of SUMO varies between SUMO family members  and in different organisms.

Function  of SUMO

Sumoylation is involved in regulating the  subcellular localization of a number of substrate proteins. Depending on the  target protein, sumoylation can occur in the cytoplasm or nucleus. For example,  transport of RanGAP1, a ribonucleoprotein, across the NPC is regulated by  addition of SUMO. Sumoylation also  regulates the transcription process. The activities of many transcription  factors are associated with interaction of PML and NBs, and assembly of PML and  NBs requires sumoylation of the PML protein. Moreover, sumoylation has held an  important role in chromosome cohesion and kinetochore assembly. Malfunction of  SUMO-1 disrupted proper distribution of chromatins into the replicated cells.

Characterization of Protein SUMOylation

Creative Proteomics has established a  highly sensitive HPLC-MS/MS platform that can analyze sumoylation in multiple  samples and in both eukaryotic and prokaryotic organisms. In addition, we have  optimized our protocol to enable more fast and sensitive site mapping service  for sumoylation analysis.

Workflow  of our sumoylation analysis service:

  • In gel or in solution digestion  of proteins
  • Enrichment of sumoylated  proteins by antibodies targeting specific sumoylated motifs (optional step)
  • HPLC separation, followed by  ESI-TOF MS/MS analysis
  • Mass spectrometry data  interpretation

Technology platform:

Creative Proteomics also provide the following bioinformatics services in Protein Post-translational Modification Analysis:
 Functional annotation and enrichment analysis
 Clustering analysis
 Network analysis
 Statistical analysis
 Proteomic analysis of post-translational modifications

Please feel free to Contact Us to discuss your projects. We hope you will find that we can meet your research needs.

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* For Research Use Only. Not for use in diagnostic procedures.
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