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Semi-quantitative Proteomics Analysis

What is Semi-quantitative Proteomics?

It is a technique used to compare protein abundances between multiple samples, without providing absolute quantification value. It aims to provide relative abundance measurements, enabling researchers to gain insights into changes in protein expression levels or post translational modifications (PTMs) under different experimental conditions. There are several approaches to perform semi-quantitative proteomics analysis, including label-based and label-free methods. Notable examples of label-based techniques include SILAC, iTRAQ/TMT, among others. For label-free approaches, protein abundance can be determined by comparing spectral counts or peptide ion intensities across samples. And we have many situations need employing semi-quantitative proteomics methods. Most importantly, it is relative cost-effective and high operability. Overall, semi-quantitative proteomics analysis provides valuable information about protein expression changes across different samples or conditions. By understanding protein regulation, researchers can gain insights into important cellular processes and disease mechanisms, contributing to biomedical research and potential therapeutic development.

The application of semi-quantitative proteomics.

1) Protein content comparison across species.

Semi-quantitative Proteomics AnalysisFigure 1. Experimental setup to study proteome-wide biological responses to non-ionizing EMFs using semi-quantitative mass spectrometry [1].

2) 2-DE /SDS-PAGE protein identification and semi-quantification by LC-MS/MS

Semi-quantitative Proteomics AnalysisFigure 2. Semi-quantification of protein on the 2-DE gel.

Semi-quantitative proteomics in Creative Proteomics

With over a decade of experience in the field of proteomics, and the field of quantitative proteomics also has witnessed the establishment of various well-established techniques, including SILAC, iTRAQ/TMT, label-free, DIA and target quantification proteomics such as (SRM/MRM, PRM). We possess exceptional proficiency in executing Semi-quantitative Proteomics Analysis Service Projects. Besides, we possess the capability to handle diverse sample types and deliver precise and comprehensive identification and quantification information regarding interacting proteins and their interaction sites.

Creative Proteomics also provides the following bioinformatics services in Semi-quantitative Proteomics Analysis:

Technologies platform

  1. Professional detection and analysis capability: Equipped with specialized proteomics and bioinformation technical team, strict quality control system, together with ultra-high resolution detection system and professional data pre-processing and analysis capability, ensure reliable and accurate data.
  2. High specificity: Optimization of experimental design and methods.
  3. High stability and reproducible: Obtain consistent and reproducible inter- and intra- assay results for data analysis.
  4. High resolution and sensitivity: Triple TOF 5600, Q-Exactive, Q-Exactive HF, Orbitrap Fusion™ Tribrid™, etc.
  5. High selectivity: We can provide a wide range of multi-technological services and efficiently handle various types of samples, while remaining cost-effective and ensuring short turnaround time for your projects.

Results Delivery

  1. Detailed report, including experiment procedures, parameters, etc.
  2. Graphical result, raw data, and data analysis results (e.g. identified proteins and peptides, and optional bioinformatics analysis, such as volcano plot, PCA, GO, KEGG, Protein Interaction Networks, etc.).

How to place an order

The provision of comprehensive support tailored to your specific requirements for Semi-quantitative Proteomics Analysis is our area of expertise. Please feel free to contact us via email whenever you need to discuss your specific requirements. Our customer service representatives are available 24 hours a day, from Monday to Sunday.

How to place an order


  1. Kuzniar A, Laffeber C, Eppink B, et al. Semi-quantitative proteomics of mammalian cells upon short-term exposure to non-ionizing electromagnetic fields. PLoS One. 2017 Feb 24;12(2): e0170762.
* For Research Use Only. Not for use in diagnostic procedures.
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