Top-Down MS is powerful for comprehensive analysis of protein modifications because Top-Down MS is able to analyze intact proteins/peptides without proteolysis. Therefore, the strategy can preserve the labile modified motifs which are mostly lost when shotgun method is applied and CID is used for fragmentation in analysis of protein therapeutics, such as monoclonal antibodies and recombinant proteins.
Top-down mass spectrometry with ECD & ETD has been successfully applied to proteomics research because of its unique advantages in intact protein/peptide mass determination, quantifying multiple modified protein forms, mapping of modifications with higher coverage, identifying unpredicted PTMs and determining the order of multiple modifications.
Although Top-Down proteomics is young and confronted with technical challenges in sample preparation, and throughput, the unique advantages mentioned above still make it a promising tech solution for characterization of post-translational modifications. Creative Proteomics have experienced scientists and technicians for Top-Down Proteomics, and are glad to provide analytical support to speed up your research.