Fatty acids are a class of carboxylic acid compounds, which are composed of hydrocarbon groups consisting of carbon and hydrogen atoms attached to carboxylic acids.
According to the length of carbon chain, fatty acids are divided into short-chain fatty acids (SCFA), medium-chain fatty acids (MCFA) and long-chain fatty acids (LCFA); according to the degree of saturation, they can be divided into saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PU-FA); unsaturated fatty acids can be divided into cis-fatty acids and trans-fatty acids according to the groups on both sides of the double bond. Unsaturated fatty acids can be divided into cis and trans fatty acids depending on the groups on both sides of the double bond. Most of the fatty acids in natural oils and fats contained in unprocessed foods are cis-fatty acids. The naturally occurring trans fatty acids are relatively small and are mainly found in the fat and milk of ruminants such as cattle and sheep. Triglycerides, formed by glycerol and chain fatty acids, are the main components of fats.
There are many analytical research methods for fatty acids, such as the common thin-layer chromatography, gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry, fourier transform infrared spectroscopy and nuclear magnetic resonance. At present, gas chromatography is the most widely used method for fatty acid analysis.
Service We Provide
Creative Proteomics uses GC/MS-based assays with a variety of fatty acid isotope standards for quantitative and qualitative analysis of over 40 fatty acids. We have significantly improved the accuracy of qualitative and quantitative analysis while greatly increasing throughput and stability. Our method has the advantages of good separation, high sensitivity, high detection limit and good stability.
Fatty Acids Analysis Service
- Omega 3 Fatty Acids
- Omega 6 Fatty Acids
- Free Fatty Acids
- Esterified Fatty Acids
- Total Fatty Acids
- TRANS Fatty Acids
- Short Chain Fatty Acids
- Medium-chain and Long-chain Fatty Acids
- Very Long Chain Fatty Acids
- Straight Chain Fatty Acids
- Branched Chain Fatty Acids
- Saturated Fatty Acids
Fatty Acids Derivatives Analysis Service
- Prostaglandins
- Leukotrienes
- Eicosanoids
- Eicosanoid Metabolites (Urine)
- CYP450 Metabolites
- Hydroxy-eicosatetraenoic Acids (HETEs)
- Lipoxygenase Products
- Endocannabinoids
- Mycolic Acids
Metabolic Pathways
- Fatty Acid Metabolism
- Oxidative stress
- Inflammatory signaling pathways
Workflow
About The Samples
Serum, plasma, urine, bile, bile acids.
Animal tissues such as cells, liver, brain tissue and feces.
plants, yeast, microorganisms, feed, etc.
1. Sample extraction
Weigh an appropriate amount of sample, add 4mL of methanol/CH2Cl2 (1:3) mixed solution, shake well; keep the temperature below 30°C for ultrasonic extraction for 10min. Take out the centrifuge tube and centrifuge in a centrifuge (1800rpm, 10min), collect the supernatant, repeat 3 times; dry the extract under gentle nitrogen flow.
2. Saponification of the extract
Add 3mL of 6%KOH methanol solution (preparation: about 6gKOH/methanol 118mL), sonicate for 10min, place for 30min, repeat 3 times, leave at room temperature overnight (cap tight) for alkaline hydrolysis; add 2mL of n-hexane, sonicate for 10min, shake Mix well, shake and centrifuge, discard the upper n-hexane extract, and repeat 3 times. Add about 1 mL of 4N HCl to the remaining solution (aqueous phase) after the above extraction to make the pH<2, and then extract with 2 mL of n-hexane for 3 times.
3. Derivatization of fatty acids
Transfer the above extract to a glass tube with a cover, dry it with nitrogen, add about 2mL of BF3-MeOH, flush the upper space of the glass tube with nitrogen, seal the cover, and heat at 90°C for 2h; after the sample is cooled, Add about 1ml of 5% NaCl solution, extract 3 times with 2ml of n-hexane, and transfer the extract to a 2mL sample bottle, dry it with nitrogen, and wait for analysis.