Arachidonic acid is known to be oxidized by several enzymatic pathways including prostaglandin H2 synthase, 5-lipoxygenase, and cytochrome P-450 dependent epoxygenase reactions. These enzymatic pathways require free arachidonic acid as substrate and result in a host of biologically active lipid mediators including prostaglandins, thromboxanes, leukotrienes, and epoxyeicosatetraenoic acids. The major arachidonic acid metabolites in blood and blood vessels are Hydroxyeicosatetraenoic acids (HETEs). HETEs have been implicated in physiologic responses such as aggregation, cell migration, and cell proliferation. GC/selected ion monitoring after high-performance liquid chromatography separation usually used as sensitive and specific assay for HETEs.
Scientists at Creative Proteomics have measured the levels of most HETE isomiers in plasma, serum and stimulated serum (formed in the presence of arachidonic acid and calcium ionophore), obtained from normal adults and cord blood from normal neonates.
HETEs could monitored in This Service
Any or all of the HETEs (5-, 8-, 9-, 11-, 12-, 15, 20-HETE) or all HETEs, HEPEs, and HDoHEs
Plasma, Tissues, Cells/Media
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.