Protein purity and homogeneity are key for protein crystallization and rigorous structural and functional studies. Impure samples will lead to inaccurate and imprecise results across experiments. In addition, Protein purity and homogeneity are two very important characteristics for further protein studies and biopharmaceutical applications. Purity of protein sample limits the application of protein products. Common contaminants of protein product include culturing medium and reagents from extraction and purification steps. In addition to impurity, protein heterogeneity may result from differential intra-cellular processing and the addition of co- and post-translational modifications. It is important to analyze the purity and homogeneity of your protein prior to using it for further processes.
Figure 1. Protein purity and homogeneity characterization using pl markers.
Overview of Protein Purity and Homogeneity Characterization Service
At Creative Proteomics, we provide a complete list of protein purity and homogeneity characterization services to detect contaminants, protein variants, isomers, mismatched S-S link, truncated proteins, degraded proteins, protein modifications, protein aggregates, and protein precursors.
- Purity and integrity: SDS-PAGE, capillary electrophoresis, Zinc-reverse staining, glutaraldehyde-free modified silver staining, MALDI-TOF mass spectrometry
- Homogeneity assay: dynamic light scattering, UV-visible fluorescence spectroscopies, size-exclusion chromatography, static light scattering
Our Technology Platforms:
- Capillary Electrophoresis Sodium Dodecyl Sulfate (CE-SDS)
- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
- Gel permeation chromatography (GPC)
- Size Exclusion Chromatography (SEC)
- Liquid Chromatography (LC)
- High Performance Liquid Chromatography (HPLC)
- Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)
- Electrospray Ionization Mass Spectrometry (ESI-MS)
- Circular Dichroism (CD)
- Nuclear Magnetic Resonance (NMR)
- Fluorescence Spectroscopy (Fluorometry)
- Dynamic Light Scattering and Static Light Scattering
Sample Requirements
We work with antibodies, antigens, enzymes, growth factors, DNA-binding proteins, blood proteins, membrane proteins, etc. We accept a range of samples, including:
- Native proteins from natural sources
- Fusion and tagged proteins from recombinant sources
- Antibodies of different isotypes
- Membrane proteins
Please submit at least 100 ug of purified protein at ≥1 mg/mL.
Advantages of Protein Purity and Homogeneity Characterization
- Advanced technical platforms. We are equipped with multiple technical platforms that allow to determine the protein purity and homogeneity accurately.
- Accuracy and reproducibility. We provide accurate and detailed results about the purity and homogeneity of your sample, which save you from wasting time on bad samples.
- A wide range of applications. Purified and homogenous proteins can be used in research and medical applications, such as the development and quality control of therapeutic proteins.
Creative Proteomics provides a wide spectrum of methods to characterize your samples cost-effectively, and delivers reliable and detailed data reports. As every project has different requirements, please contact our specialists to discuss your specific needs.
Reference
1. Raynal B, Lenormand P, Baron B, et al. Quality assessment and optimization of purified protein samples: why and how?. Microbial cell factories, 2014, 13(1): 180
