Biacore system is based on the principle of SPR (surface plasmon resonance) and is commonly used to study biomolecular interactions in real time. The platform can provide detailed information about bispecific binding interactions and kinetic studies without the use of labeled molecules.
Technology Platform of Biacore Service:
The Biacore assay involves the immobilization of one interacting molecule (ligand) on the surface of the sensor chip and the injection of the sample containing other interaction partners (analytes) with a series of concentrations over the surface. The sample is carried in a buffer with continuous and controlled flow. The response generated from the binding of analytes on the sensor surface is proportional to the bound mass which corresponds to the concentrations of the analytes in the sample solution.
The response curve of the system is displayed during the course of analysis. The change of resonance is very sensitive to the changes at the surface of the sensor chip. The sensor chip is a vital part of Biacore platform which determines the sensitivity of the system. The molecules can be immobilized on the surface of the sensor chip through different approaches such as high affinity capture (non-covalent interaction), covalent chemical interaction, and hydrophobic adsorption. The sensor chip has different functions to meet the needs and requirement of different projects.
The approaches for capturing the target analyte rely on the interaction between the analyte and the ligand on the sensor surface. Some common capturing approaches are streptavidin/ avidin-biotin interaction, antibody-antigen interaction, tagged recombinant proteins based capturing.
Figure 1. The principle of Biacore (SPR). Schematic representation of a sensorgram curve when the sample solution pass over the sensor surface
We Can Provide but Not Limited to:
Biacore platform has a wide variety of applications in protein interactions:
- Investigate the affinity and kinetics of an interaction by testing the binding behavior of the molecular pairs
- Study the specificity of an interaction by analysis of the binding between different molecule pairs
- Measure the active concentration of specific molecules in the sample
- Study the immunogenicity and thermodynamic parameters
Advantages of Biacore Service:
- High sensitivity: The detection limit can be as low as picomolar to nanomolar range for the analyte.
- High specificity: The specificity is determined by the properties of the biomolecules attached to the sensor surface.
- Label-free analysis: It does not require the analytes to be labelled with a colorimetric or fluorescent label.
- Real-time monitoring: The interaction between the ligand and analyte resulting in the change of the sensor surface can be monitored in real time.
- Customized service: We can customize the service based on the needs of your research plan and provide professional solutions to you. You can determine or recommend the required items for analysis.
Creative Proteomics's research team can provide customers with professional and high-quality Biacore service. We will provide you with an accurate and sensitive Biacore platform, analysis reports, and other data collection and processing process related experimental reports. We are committed to providing you with expertise in a variety of analyses to help you solve problems related to technology and analysis.
Reference
- Jason-Moller, L., Murphy, M., & Bruno, J. (2006). Overview of Biacore Systems and Their Applications. Current protocols in protein science, 45(1), 19-13.
- Fivash, M., Towler, E. M., & Fisher, R. J. (1998). BIAcore for macromolecular interaction. Current opinion in biotechnology, 9(1), 97-101.
- Malmqvist, M. (1999). BIACORE: an affinity biosensor system for characterization of biomolecular interactions. Biochemical society transactions, 27(2), 335-340.
- Myszka, D. G. (2000). Kinetic, equilibrium, and thermodynamic analysis of macromolecular interactions with BIACORE. Methods in enzymology, 323, 325-340.
