Biacore system is based on the principle of SPR (surface plasmon resonance) and is commonly used to study biomolecular interactions in real time. The platform can provide detailed information about bispecific binding interactions and kinetic studies without the use of labeled molecules.
The Biacore assay involves the immobilization of one interacting molecule (ligand) on the surface of the sensor chip and the injection of the sample containing other interaction partners (analytes) with a series of concentrations over the surface. The sample is carried in a buffer with continuous and controlled flow. The response generated from the binding of analytes on the sensor surface is proportional to the bound mass which corresponds to the concentrations of the analytes in the sample solution.
The response curve of the system is displayed during the course of analysis. The change of resonance is very sensitive to the changes at the surface of the sensor chip. The sensor chip is a vital part of Biacore platform which determines the sensitivity of the system. The molecules can be immobilized on the surface of the sensor chip through different approaches such as high affinity capture (non-covalent interaction), covalent chemical interaction, and hydrophobic adsorption. The sensor chip has different functions to meet the needs and requirement of different projects.
The approaches for capturing the target analyte rely on the interaction between the analyte and the ligand on the sensor surface. Some common capturing approaches are streptavidin/ avidin-biotin interaction, antibody-antigen interaction, tagged recombinant proteins based capturing.
Figure 1. The principle of Biacore (SPR). Schematic representation of a sensorgram curve when the sample solution pass over the sensor surface
Biacore platform has a wide variety of applications in protein interactions:
Creative Proteomics's research team can provide customers with professional and high-quality Biacore service. We will provide you with an accurate and sensitive Biacore platform, analysis reports, and other data collection and processing process related experimental reports. We are committed to providing you with expertise in a variety of analyses to help you solve problems related to technology and analysis.
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