Overview of Post-translational Modification Analysis

Post-translational  modification (PTM) refers to the covalent, usually enzymatic modification of  proteins, and protein process during or after protein biosynthesis. Protein  post-translational modification (PTM) increases the functional diversity of the  proteome by the modifying proteins with functional groups, such as phosphate,  acetate, amide groups, or methyl groups, and influences almost all the aspects  of normal cell biology and pathogenesis. It plays a key role in many cellular  processes such as cellular differentiation, protein degradation, signaling and  regulatory processes, regulation of gene expression, and protein-protein  interactions. The modifications genererally include phosphorylation,  glycosylation, ubiquitination, nitrosylation, methylation, acetylation,  lipidation and proteolysis and influence almost all aspects of normal cell  biology and pathogenesis. Therefore, characterization of PTM, including the  modification categories and modified sites, is critical in the study of cell  biology and disease diagnostics and prevention.

Identification  of Post-Translational Modifications (PTM) is a tedious process. It can be  affected by many factors. For example, most of the post-translational  modifications are present in very low level. Therefore, enrichment steps are  necessary before identification process. Additionally, stability of  modification, and detection efficiency of mass spectrometry are also critical  factors in PTM identification process. Creative Proteomics offers an advanced  analytical platform for the characterization of various post-translational  modifications (PTM).


Function of PTMs:

Category of PTMsFunctions  
Phosphorylation Change protein comformation; Activate/inactivate catalytic activity
Ubiquitination Target protein for degradation
Glycosylation Direct a protein to its destination
Acetylation  Affect protein comformation and its affinity to other proteins
Lipidation Affect the activity and subcellular location of a protein
Methylation Affect protein comformation and its affinity to other proteins
Proteolysis Remove peptide sequences or regulatory domains

Types of PTMs  analysis service we provide:

Workflow of our  PTMs analysis service:

Technology platform:

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Ordering Procedure

* For Research Use Only. Not for use in diagnostic procedures.
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