Propionylation Analysis Service

Gene expression in eukaryotes is tightly regulated, on the one hand by transcription factors; on the other hand, it is regulated at the epigenetic level. Histone modifications are an important part of epigenetics, and with the rapid development of high-resolution mass spectrometry, novel histone modifications can be identified. Among them, lysine propionylation modification is an important reversible post-translational modification of histone proteins discovered in recent years, which is widely involved in a series of biological processes and cellular metabolic pathways in prokaryotes and eukaryotes. In addition, propionylation modification has also been found to play an important role in the overall metabolic regulation network of bacteria and the activity regulation of metabolic enzymes, the cellular stress response of bacteria and mammals, the epigenetic regulation of eukaryotes and prokaryotes, as well as the regulation of photosynthesis.

Propionylation is the process of covalent binding propionyl groups (e.g., propionyl coenzyme A and other donors) to lysine residues in substrate proteins catalyzed by the action of acyltransferases (e.g., acetyltransferases).

Our Propionylation Proteomics Service

Creative proteomics offers the lysine propionylation modification service to identify propionylated proteins and their modification sites using high-resolution tandem mass spectrometry complementing the enrichment strategy.

Workflow

Customized Bioinformatics Services

• Modified proteins and modification sites identification
• Characterize identification results: peptide length, protein coverage distribution, unique peptide distribution
• Modification site motif prediction
• Protein GO functional classification, COG annotation, Pathway pathway annotation
• Protein network interactions prediction (advanced analysis)
• Protein domain prediction (advanced analysis)
• Protein subcellular localization (advanced analysis)

Sample requirements

• Fresh animal tissue: ≥600 mg
• Fresh plant tissue: ≥6 g
• Cell culture: ≥1×10⁷ cells/tube x 3 tubes
• Fungi and bacteria: ≥600 mg
• Serum, plasma: 450 μL × 4 tubes
• Protein solution: total protein of 5-10 mg
• Body fluid samples: urine of 15 mL × 4 tubes (centrifuge at 1000 x g for 5 minutes and discard sediment); or other body fluids (saliva, amniotic fluid, cell culture supernatant, etc.) > 15 mL

Advantages

• High specificity and enrichment efficiency
• Large-scale identification of enriched propionylated peptides with mass spectrometry of high resolution and high throughput
• Combining commercially available quantitative techniques to analyze, compare, and correlate propionylation levels between samples quantitatively

Applications

• Cellular stress response
• Bacterial metabolic regulatory networks
• Photosynthesis
• Epigenetic regulation

Technology platform

Ion Chromatography
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)

Reference
1. Adam F Kebede, et al. Histone propionylation is a mark of active chromatin. Nature structural & molecular biology.(2017).