Acylation is one type of post-translational modification of proteins. Acyl-CoA, an important intermediate metabolite of cells, directly participates in the protein acylation in organisms as donors, thereby regulating various important biological processes, including epigenetics, energy metabolism, protein transport, protein-protein interaction, etc. Meanwhile, protein acylation is one of the most attractive topics in current life science research. Among protein modifications, acetylation modification is the earliest discovered and most studied, and in recent years, protein propionylation, malonylation, glutarylation, succinylation, crotonylation, and other acylations have been discovered to broaden research proteomics. Acylation quantitative proteome research is to compare the acylation modification levels in biological samples under different physiological or pathological conditions and to reveal the relationships between changes of acylation modification of protein and life activities, which is useful for elucidating the mechanism of cells, screening clinical markers of diseases, and discovering novel drug targets for future treatments.
Creative Proteomics relies on Thermo Fisher's Orbitrap Fusion Lumos Mass Spectrometry platform combined with nanoLC-MS/MS to provide acylated quantitative proteomic analytical service packages. We will take care of all aspects of the project, covering protein extraction, proteolytic cleavage, acylated peptide enrichment, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis.
During our service cycle, your provided protein samples will be digested into a mixture of peptide fragments and then separated by liquid chromatography according to their different components, hence reducing the overall complexity of your protein sample. Subsequently, acylated peptides are annotated, identified, and specifically enriched using the high-quality acylated modified antibodies or kits for final bioinformatic analysis. As a result, the acylated protein modification are revealed, analyzed, and quantified by the LC-MS/MS.
|Standard Analysis||Customized Analysis|
a) Collection of output statistics
Modified peptide motif analysis
- High specificity and enrichment efficiency
- Large-scale identification of enriched acylated peptides with mass spectrometry of high resolution and high throughput
- Combining commercially available quantitative techniques to analyze, compare, and correlate acylation levels between samples quantitatively