Acylation Quantitative Proteomics Service

Acylation is one type of post-translational modification of proteins. Acyl-CoA, an important intermediate metabolite of cells, directly participates in the protein acylation in organisms as donors, thereby regulating various important biological processes, including epigenetics, energy metabolism, protein transport, protein-protein interaction, etc. Meanwhile, protein acylation is one of the most attractive topics in current life science research. Among protein modifications, acetylation modification is the earliest discovered and most studied, and in recent years, protein propionylation, malonylation, glutarylation, succinylation, crotonylation, and other acylations have been discovered to broaden research proteomics. Acylation quantitative proteome research is to compare the acylation modification levels in biological samples under different physiological or pathological conditions and to reveal the relationships between changes of acylation modification of protein and life activities, which is useful for elucidating the mechanism of cells, screening clinical markers of diseases, and discovering novel drug targets for future treatments.

Our Protein Acylation Analysis Service

Creative Proteomics relies on Thermo Fisher's Orbitrap Fusion Lumos Mass Spectrometry platform combined with nanoLC-MS/MS to provide acylated quantitative proteomic analytical service packages. We will take care of all aspects of the project, covering protein extraction, proteolytic cleavage, acylated peptide enrichment, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis.

Workflow of Our Protein Acylation Analysis Service

During our service cycle, your provided protein samples will be digested into a mixture of peptide fragments and then separated by liquid chromatography according to their different components, hence reducing the overall complexity of your protein sample. Subsequently, acylated peptides are annotated, identified, and specifically enriched using the high-quality acylated modified antibodies or kits for final bioinformatic analysis. As a result, the acylated protein modification are revealed, analyzed, and quantified by the LC-MS/MS.

Workflow of Our Protein Acylation Analysis Service

Customized Bioinformatics Services

Standard Analysis	Customized Analysis
a) Collection of output statistics b) Establishment of database and search for modified proteins c) Identification of modified proteins and modification sites d) Annotation of modified proteins e) Quantitative analysis of modified proteins f) Analysis of differential expression of modified proteins g) Functional enrichment analysis of differentially modified proteins GO/KEGG	Modified peptide motif analysis Protein network interaction prediction Protein domain prediction (Advanced Analysis) Protein subcellular localization analysis (Advanced Analysis)

Sample requirements

Fresh animal tissue: ≥600 mg
Fresh plant tissue: ≥6 g
Cell culture: ≥1×10⁷ cells/tube x 3 tubes
Fungi and bacteria: ≥600 mg
Serum, plasma: 450 μl × 4 tubes
Protein solution: total protein of 5-10 mg
Body fluid samples: urine of 15 mL × 4 tubes (centrifuge at 1000 x g for 5 minutes and discard sediment); or other body fluids (saliva, amniotic fluid, cell culture supernatant, etc.) > 15 mL

Advantages

- High specificity and enrichment efficiency

- Large-scale identification of enriched acylated peptides with mass spectrometry of high resolution and high throughput

- Combining commercially available quantitative techniques to analyze, compare, and correlate acylation levels between samples quantitatively

Types of Our Protein Acylation Analysis Service

Propionylation Quantitative Proteomics
Malonylation Quantitative Proteomics
Glutarylation Quantitative Proteomics
Succinylation Quantitative Proteomics
Crotonylation Quantitative Proteomics
Palmitoylation Site Identification Service

Technology platforms

Ion Chromatography
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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