Sphingomyelin profiling service provides data on all individual molecular species. Sphingomyelins (SM) constitute an important class of phospholipids in the membranes of most eukaryotic cells. In mammalian tissues, SM usually constitute 2–15% of the total organ phospholipid, but certain tissues such as brain, peripheral nervous tissue and ocular lenses have even higher SM contents, especially in some nerve cell axons. Sphingomyelin (or ceramide phosphorylcholine) consists of a ceramide unit with a phosphorylcholine moiety attached. Sphingomyelin does not appear to occur in plants or fungi, which produce ceramide phosphorylinositol instead, or in bacteria with rare exceptions, and its evolutionary significance is a matter for speculation.
Sphingomyelin profiling service provides data on all individual molecular species. We recommend these profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. Sphingomyelin usually isolated from animal tissues by TLC or HPLC. The peaks or bands usually split into two or three resolved fractions, due to the changes in hydrophobicity resulting from the wide range of chain lengths in the fatty acid constituents, or to the presence of 2-hydroxy acids. Molecular species of the intact lipid can be resolved by reversed-phase HPLC, but another useful approach is to hydrolyse to the less polar ceramides with the enzyme phospholipase C, analysed the ceramides instead. Sphingomyelin profiling service at Creative Proteomics ESI Liquid Chromatography Mass Spectrometry (ESI- LC/MS) is used for the analysis of sphingomyelin and other sphingo-phospholipids.
- ESI Liquid Chromatography Mass Spectrometry (ESI- LC/MS)
- Cultured Cells
- Cultured or uncultured primary mouse cells or cell lines
|Sphingomyelins could monitored in This Service|
|14:0 SM||15:0 SM||16:1 SM|
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|20:1 SM||20:0 SM||22:1 SM|
|22:0 SM||23:0 SM||24:2 SM|
|24:1 SM||24:0 SM||More on Request|
With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.