Propionylation Analysis Service

Uncover how lysine propionylation reshapes chromatin and metabolism. Creative Proteomics delivers site-specific Kprop profiling through validated enrichment, high-resolution LC–MS/MS, and advanced bioinformatics—turning modification data into mechanistic insight.

Site-level Kprop identification & quantification with strict FDR
Anti-propionyl-lysine enrichment and Orbitrap ETD/EThcD/HCD precision
Flexible DIA/TMT/PRM workflows for discovery to targeted analysis
Integrated motif, pathway, and network bioinformatics
Reproducible, decision-ready reports for research use only

Submit Your Request Now

Submit Your Request Now

Define
What We Provide
Technology Platform
Workflow
Advantages
Applications
Sample Requirement
Demo
FAQs
Case
Publications

What Is Propionylation?

Propionylation modification is an important post-translational modification (PTM) in which the propionyl group derived from propionyl-CoA is covalently attached to protein lysine residues through the catalysis of propionyl transferase, therby changing the charge of lysine from +1 to 0. Similar to acetylation, propionylation affects the protein's functional activity by altering its charge, spatial structure, and interaction ability. The propionylation of lysine was first discovered to exist in histones, and later the propionylation of non-histone substrates in mammals was also demonstrated. It is dynamically regulated by specific propionyltransferases and depropionylases. Propionylation modification plays a significant role in key biological processes such as gene expression regulation, metabolic adaptation, and cell signaling.

Propionylation Service at Creative Proteomics

Lysine propionylation is a novel post-translational modification essential for epigenetic regulation and metabolic homeostasis^[1]. It alters nucleosome structure, modulates gene transcription, and regulates key metabolic enzymes in glycolysis and the TCA cycle. Abnormal propionylation is linked to diseases such as cancer and neurodegeneration, making it a promising biomarker and therapeutic target. We provide comprehensive propionylation proteomics services, including high-resolution MS detection, site-specific identification, quantitative profiling, and bioinformatics analysis, helping researchers uncover functional roles, pathway associations, and clinical relevance of this emerging modification.

Propionylation Research Platforms

Easy-nLC 1000 nanoliter liquid chromatography system

Easy-nLC 1000 (Figure from Thermo Scientific)

Thermo Fisher LTQ Orbitrap ETD mass spectrometer, combined with linear ion trap and Orbitrap technology, supports ETD fragmentation mode

Thermo Fisher LTQ Obitrap ETD (Figure from Thermo Scientific)

Workflow of Propionylation Analysis

Advantages Our Propionylation Service

Professional detection and analysis capability: Experienced research team, strict and matured techniques.
Breadth: we could skillfully deal with protein samples derived from a wide range of sources, encompassing cell, animal and plant tissues, microorganism, etc.
High specificity and accuracy: Skillful quantification proteomics techniques, and PTMs enrichment methods.
High stability and reproducible: Obtain consistent and reproducible inter- and intra- assay results for data analysis.
Advanced Analytical Platforms: Use of high-sensitivity mass spectrometry and state-of-the-art tools, ensuring precise, repeatable results.
End-to-End Support: Offer full-spectrum services from design to data interpretation, with continuous technical support throughout the research process.

Applications of Propionylation

Tumor

Propionylation modification affects tumor metabolic reprogramming and epigenetic alterations by regulating histone function and non-histone activity, and can serve as a tumor diagnostic marker and therapeutic target^[2].

Neurodegenerative disease

Propionylation modification is abnormally upregulated in the rat model of vascular dementia, while the drug donepezil can exert neuroprotective effects by down-regulating the levels of propionylation and other acylation modifications, suggesting that targeted protein propionylation regulation may be a new strategy for treating neurodegenerative diseases^[3].

Cardiometabolic health

Abnormal changes in protein propionylation modification are closely related to the occurrence and development of atrial fibrillation after cardiac surgery, suggesting its potential application value in the diagnosis and treatment of cardiovascular diseases^[4].

Propionylation Service Sample Requirements

Sample	Sample Quantity
Tissue	animal tissue	200-500mg
Tissue	fresh plant	200-500mg
Cell	suspension cell	> 3x10⁷
	adherent cell	> 3x10⁷
	microorganism	> 50 mg or 3 × 10⁷ cells
Protein	Total protein > 5mg and concentration >1 μg/μL

Demo Result of Propionylation Service

Bar plot

PCA plot

Heat map

GO analysis

Heatmap

The plot is used to show the enrichment of propionylated proteins in different pathways.

KEGG analysis

The protein-protein interaction network demonstrated the interactions between all propionylation modification differential proteins.

PPI

FAQs of Propionylation Service

Which sample types are accepted by the service？

We accept a variety of sample types including cells, tissues,, plant tissue and more. If you have other special sample types, please consult in advance.

Whether the sample needs pretreatment？

We recommend that customers provide fresh or frozen samples, which should avoid repeated freezing and thawing, and we will be responsible for subsequent protein extraction and pretreatment. If you have already extracted the protein, please communicate with us in advance about the sample handling method.

Learn about other Q&A.

Case Study

Article

Publications

Here are some publications in Proteomics research from our clients:

Pressure overload induces ISG15 to facilitate adverse ventricular remodeling and promote heart failure. 2023. https://doi.org/10.1172/JCI161453
3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture. 2019. https://doi.org/10.1016/j.biomaterials.2018.08.057
Assessment of Fasciola hepatica glutathione S-transferase as an antigen for serodiagnosis of human chronic fascioliasis. 2018. https://doi.org/10.1016/j.actatropica.2018.07.002
Characterizing the proteome of bullous pemphigoid blister fluid utilizing tandem mass tag labeling coupled with LC–MS/MS. 2022. https://doi.org/10.1007/s00403-021-02253-8
Parkinson’s disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes. 2019. https://doi.org/10.1016/j.yjmcc.2007.03.738

More Publications

References

Lagerwaard, B., Pougovkina, O., Bekebrede, A. F., Te Brinke, H., Wanders, R. J. A., Nieuwenhuizen, A. G., Keijer, J., & de Boer, V. C. J. (2021). Increased protein propionylation contributes to mitochondrial dysfunction in liver cells and fibroblasts, but not in myotubes. Journal of inherited metabolic disease, 44(2), 438–449. https://doi.org/10.1002/jimd.12296.
Fu, Y., Yu, J., Li, F., & Ge, S. (2022). Oncometabolites drive tumorigenesis by enhancing protein acylation: from chromosomal remodelling to nonhistone modification. Journal of experimental & clinical cancer research : CR, 41(1), 144. https://doi.org/10.1186/s13046-022-02338-w
Wang, H., Lu, J., Gao, W. C., Ma, X., Li, N., Ding, Z., Wu, C., Zhu, M., Qiao, G., Xiao, C., Zhang, C., Chen, C., Weng, Z., Yang, W., & Zheng, C. B. (2020). Donepezil down-regulates propionylation, 2-hydroxyisobutyrylation, butyrylation, succinylation, and crotonylation in the brain of bilateral common carotid artery occlusion-induced vascular dementia rats. Clinical and experimental pharmacology & physiology, 47(10), 1731–1739. https://doi.org/10.1111/1440-1681.13352
Zhang, Y., Chen, H. X., Hou, H. T., Liu, Y. Q., Chen, Z., Yang, Q., & He, G. W. (2025). Atlas of lysine propionylation of human right atrium and ALDH6A1-NADH pathway in new-onset atrial fibrillation after coronary surgery. Communications biology, 8(1), 858. https://doi.org/10.1038/s42003-025-08264-9

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry

From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

Great Minds Choose Creative Proteomics