Single-Nucleus RNA Sequencing (snRNA-seq) Service

Single-Nucleus RNA Sequencing (snRNA-seq) is an advanced technique that enables the analysis of gene expression at the level of individual cell nuclei. Unlike traditional single-cell RNA sequencing (scRNA-seq), which requires intact cells, snRNA-seq isolates and analyzes the RNA from cell nuclei, making it ideal for studying tissue samples where whole cells are difficult to isolate, such as in brain tissue or frozen samples. This method provides valuable insights into transcriptional activity, even in complex or heterogeneous cell populations, allowing researchers to explore cellular dynamics with high precision.

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graphical abstract of Single-Nucleus RNA Sequencing (snRNA-seq)

Workflow
Platforms
Deliverables
Advantages
Who will choose
Why choose
FAQs
Sample preparation

Workflow

snRNA-seq workflow

Platform

We utilize advanced sequencing platforms like Illumina NovaSeq and 10x Genomics Chromium to ensure high-quality, reliable results. These technologies are optimized for single-nucleus RNA sequencing, enabling us to achieve deep coverage and high sensitivity.

Deliverables

Raw Data: High-throughput sequencing reads.
Processed Data: Cleaned, aligned data ready for downstream analysis.
Gene Expression Profiles: Quantitative expression levels for thousands of genes across individual nuclei.
Custom Analysis Reports: Tailored bioinformatics analysis to match your research needs.

Advantages

Ideal for tissues that are challenging to dissociate into single cells: Perfect for samples like brain tissue, where isolating intact cells is highly difficult.
Preserves Nucleus-Related Information: Offers insights into transcriptional activity at the nucleus level, essential for certain research applications.
High Sensitivity: Provides deep sequencing of gene expression data, even from low-abundance transcripts.
Cellular Heterogeneity Analysis: Capable of identifying subpopulations within complex tissues, facilitating more accurate cell-type discovery.

Who Is This Service Suitable For?

This service is ideal for researchers and labs facing specific challenges in gene expression analysis. Key problems solved by Single-Nucleus RNA Sequencing (snRNA-seq) include:

Tissues with Difficult Cell Isolation:
For samples where isolating intact cells is challenging (e.g., brain tissue, frozen tissues), snRNA-seq allows analysis at the nucleus level without requiring intact cells.
Analysis of Rare or Heterogeneous Cell Populations:
Ideal for studying complex tissues with mixed cell types, where distinguishing subpopulations is crucial for understanding cellular diversity and function.
Studies on Frozen Samples:
snRNA-seq is well-suited for analyzing frozen samples, where traditional cell isolation methods may not be feasible.
Cellular Mechanism Studies
Enables deeper understanding of transcriptional regulation at the nuclear level, especially in research on chromatin states and nuclear activity.

Why Choose Our Service?

Expertise: With years of experience in single-cell and single-nucleus sequencing, we provide tailored solutions for a variety of research needs.
High Quality: We use top-tier platforms and stringent quality controls to ensure the accuracy and reproducibility of your data.
Comprehensive Support: From sample preparation to data analysis, our team provides expert assistance throughout the entire process.
Customized Solutions: We understand that every project is unique—our service is adaptable to your specific research requirements.

FAQs

What is the difference between snRNA-seq and scRNA-seq?

snRNA-seq focuses on the analysis of gene expression at the nucleus level, whereas scRNA-seq examines the entire transcriptome of whole cells. snRNA-seq is ideal for tissue samples where obtaining intact cells is challenging.

How much tissue do I need to send?

Typically, we require 20-50 mg of fresh or frozen tissue. However, the exact amount may vary depending on the tissue type and your specific project.

Can you help with data interpretation?

Yes, we offer comprehensive bioinformatics support to help you interpret the sequencing data. Custom analysis packages are available to suit your research needs.

Learn about other Q&A.

Sample Submission Guidelines

Sample Type	Required Amount	Recommended Storage
Frozen Tissue	20-50 mg	-80°C
Fresh Tissue	20-50 mg	Fresh or on ice
Cell Suspension	1-2 million cells	On ice

Note: For detailed submission instructions, please contact our technical team.

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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