Single Cell Sorting and Isolation Service

Achieving high-quality single-cell data begins with precise and gentle cell isolation. Our Single Cell Sorting and Isolation Service provides researchers with customized, end-to-end solutions to obtain highly pure and viable single cells from diverse and complex biological samples. Whether you are conducting single-cell RNA sequencing (scRNA-seq), characterizing the tumor microenvironment, or studying rare cell populations, our optimized protocols ensure your starting material is of the highest quality for any downstream multiomic application.

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overview of Digital & Molecular Pathology Service

Our services
Workflow
Platform
Applications
Why Choose
FAQs

Our Services

We offer a comprehensive suite of sorting and isolation techniques tailored to your specific sample type and research goals:

Manual Sorting: Ideal for extremely rare or highly sensitive cells. We utilize precise micromanipulation to visually identify and isolate individual cells, ensuring maximum viability with minimal mechanical stress.
Immunomagnetic / Magnetic Bead-Based Cell Isolation: A highly efficient and gentle method for bulk enrichment or depletion of specific cell populations. By utilizing antibody-coupled magnetic beads, we can rapidly process large volumes of cells while maintaining excellent cell health.
Flow Cytometry-based Sorting (FACS / Flow Sorting): Our advanced FACS services provide high-throughput, multiparametric sorting. We can isolate specific, complex subpopulations based on intracellular or surface markers with exceptional purity, yield, and speed.
Primary Cells from Solid Tissues: We specialize in the complex enzymatic and mechanical dissociation of solid tissues (e.g., tumors, brain, liver, skin) into single-cell suspensions. Our optimized protocols clear debris and dead cells, maximizing the recovery of viable primary cells.
BD Rhapsody™ Single-Cell Analysis System: We offer comprehensive services utilizing the BD Rhapsody platform for high-throughput single-cell capture and molecular indexing. This enables targeted or comprehensive transcriptome analysis paired with multiplexed protein detection (AbSeq) at the single-cell level.

Workflow

Single Cell Sorting and Isolation Service workflow

Platform

Our laboratories are equipped with industry-leading technologies to guarantee precision and reproducibility:

Flow Cytometers: State-of-the-art cell sorters equipped with multiple lasers for complex, multi-color panel designs.
Magnetic Separators: Automated and manual magnetic bead isolation systems for scalable processing.
Tissue Dissociation Systems: Automated gentle tissue dissociators for reproducible primary cell extraction.
Single-Cell Capture: BD Rhapsody™ Express Single-Cell Analysis System for highly multiplexed transcriptomic and proteomic library preparation.
Cell Counters: Automated fluorescent cell viability counters for precise QC.

Applications

Single-Cell Multiomics: scRNA-seq, scDNA-seq, and CITE-seq.
Oncology: Profiling the tumor microenvironment (TME) and identifying rare cancer stem cells or circulating tumor cells (CTCs).
Immunology: Deep phenotyping of immune cell subsets and discovering novel immune biomarkers.
Stem Cell Research: Isolating specific progenitor populations for differentiation studies.
Neuroscience: Dissociating and sorting delicate neural cells from brain tissue.

Why Choose Us

Uncompromising Viability & Purity: Our protocols are continuously optimized to minimize cell stress and maximize target purity, ensuring your downstream assays succeed.
Deep Scientific Expertise: Our team consists of experienced scientists who understand the unique handling requirements of delicate primary tissues and rare cell populations.
Customizable Solutions: We do not believe in one-size-fits-all. Every project is tailored to your specific sample constraints and data requirements.
End-to-End Capability: From raw solid tissue to publication-ready data, we can handle your entire single-cell workflow under one roof.

FAQs

What types of starting materials do you accept?

We accept a wide variety of samples, including whole blood, PBMCs, cultured cell lines, bone marrow, and various solid tissues (human and animal models). If you have a unique sample type, please reach out for a custom consultation.

Can you process the isolated cells for sequencing directly?

Yes. Through platforms like the BD Rhapsody, we can take your isolated cells directly into single-cell capture and library preparation, providing you with sequence-ready material or final data.

How should I ship my samples to your facility?

Shipping conditions are highly dependent on your specific sample type. Cultured cells, blood, or fresh tissues for dissociation are typically shipped overnight in specialized preservation buffers on wet ice. Cryopreserved cells must be shipped on dry ice. We will provide you with a detailed, customized shipping protocol and buffer recommendations upon project initiation to guarantee sample integrity upon arrival.

Learn about other Q&A.

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

Great Minds Choose Creative Proteomics