Single-Cell Full-Length RNA Sequencing (Smart-Seq) Service
Unlock the full potential of single-cell gene expression analysis with our Single-Cell Full-Length RNA Sequencing (Smart-Seq) service. Unlike conventional single-cell RNA sequencing (scRNA-seq), which typically captures only partial sequences of mRNA (such as the 3' or 5' end), Smart-Seq allows for the sequencing of full-length mRNA transcripts from individual cells. This provides a more complete and detailed profile of gene expression, enabling the discovery of alternative splicing events, novel isoforms, and low-abundance transcripts that may be missed with other methods.
While conventional scRNA-seq is often used for profiling general gene expression patterns, Smart-Seq Full-Length scRNA-seq offers several key advantages:
- Comprehensive Data: Smart-Seq captures the entire mRNA sequence, allowing for the analysis of alternative splicing, novel transcript isoforms, and detailed gene structures. Traditional scRNA-seq methods often only capture a portion of the transcript, which can limit the information obtained.
- Better Isoform and Splicing Detection: The full-length coverage allows for the identification and quantification of gene isoforms and splicing variants, which is particularly important in understanding complex regulatory mechanisms in diseases like cancer and neurological disorders.
Whether you are investigating cell heterogeneity, gene regulation, or disease mechanisms, our Smart-Seq Full-Length scRNA-seq service provides the most accurate and detailed single-cell transcriptomic data available.
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- Workflow
- Deliverables
- Applications
- Why choose
- FAQs
- Sample preparation
Workflow
Deliverables
- Raw Sequencing Data
- Gene Expression Profiles
- Transcript Isoform Quantification
- Bioinformatics Report
Applications
- Cell Heterogeneity Studies
Identify and profile diverse cell populations within heterogeneous tissues.
- Gene Isoform and Splicing Analysis
Study alternative splicing events and uncover novel isoforms that could be linked to disease states.
- Developmental Biology
Trace dynamic gene expression changes during development, cell differentiation, and stem cell differentiation.
- Cancer Research
Analyze tumor microenvironment cell populations and their gene expression profiles, aiding in the discovery of cancer biomarkers.
- Neuroscience
Explore neuronal diversity, gene expression in synaptic plasticity, and neurodegenerative disease mechanisms at the single-cell level.
Why Choose Our Smart-Seq Service?
- High Sensitivity & Precision: Capture both abundant and low-expressed genes for a more complete transcriptional profile of each single cell.
- Comprehensive Data: Unlike partial sequencing methods, our service provides full-length mRNA data, enabling deeper insights into gene regulation and alternative splicing.
- Tailored Bioinformatics: Our expert bioinformaticians deliver customized analysis to ensure the most relevant and actionable results for your research goals.
- Experienced Support: Benefit from our extensive experience in handling a wide range of biological samples and delivering high-quality sequencing data.
FAQs
What types of samples can be used for Smart-Seq?
We accept a variety of sample types including tissue, blood, organoids, and cultured cells. Please contact us if you have specific requirements for your sample.
Can I get raw sequencing data?
Yes, we provide raw sequencing data (FASTQ files) as part of the deliverables, along with processed data and reports.
What is the difference between Smart-Seq Full-Length scRNA-seq and conventional scRNA-seq?
Smart-Seq Full-Length scRNA-seq captures the entire mRNA transcript, allowing for detailed analysis of alternative splicing and isoforms, providing a more comprehensive view of gene expression. In contrast, conventional scRNA-seq typically captures only the 3' or 5' end of the mRNA, offering a less detailed profile. Smart-Seq is ideal for studies requiring high-resolution data, including low-expression genes and splicing events, but it is more costly and complex compared to the simpler, more affordable normal scRNA-seq, which is suited for broad gene expression profiling.
Learn about other Q&A.
Sample Submission Guidelines
| Sample Type | Preparation | Shipping |
|---|---|---|
| Cell Samples | Single-cell suspension (1,000 cells/μL minimum) | Ship in chilled transport medium with ice packs |
| Tissue Samples | Flash-freeze or store in RNA preservative (e.g., RNAlater) | Ship on dry ice |
| General Notes | Ensure secure packaging and overnight shipping for sample integrity. | Contact us for special handling needs |
Note: Please contact us prior to sample preparation to determine the most suitable preparation protocol and SOP, in order to avoid sample loss.
