Spatial Targeted Proteomics Service
Precision Mapping with Spatial Targeted Proteomics
Our Spatial Targeted Proteomics service offers a highly specific and sensitive analysis of defined protein panels tailored to your research needs. Whether you are validating a small set of biomarkers or mapping a complex signaling pathway, this customized service allows you to select the exact targets you need directly within tissue sections. This technique combines the power of multiplexed mass spectrometry (MS) with spatially resolved tissue samples to provide a detailed understanding of protein localization, expression, and interaction in their native tissue context. Whether you are studying cancer, neurodegenerative diseases, or any other tissue-specific pathology, our service provides crucial insights into the spatial distribution of biomarkers, signaling pathways, and other key proteins of interest.
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- Workflow
- Platform
- Demo Report
- Applications
- Why choose
- FAQs
- Sample preparation
Workflow
Technology Platform
Depending on your target protein's abundance and the complexity of your tissue, we deploy the optimal mass spectrometry platform to ensure maximum sensitivity and specificity.
1. High-Resolution Q-TOF (Specificity Focus)
Technology: Parallel Reaction Monitoring (PRM) via Bruker timsTOF.
Best For: Distinguishing isoforms, mutations, and post-translational modifications (PTMs).
Advantage: The ultra-high resolution (>240,000 FWHM) allows us to separate your target peptide from complex background noise with sub-ppm accuracy, ensuring that the signal you see is truly your protein of interest.
2. Triple Quadrupole (QqQ) / QTRAP (Sensitivity Focus)
Technology: Multiple Reaction Monitoring (MRM) via Thermo TSQ systems.
Best For: Absolute quantification of low-abundance proteins and signaling molecules.
Advantage: Known as the "gold standard" for quantitation, MRM filters ions twice (precursor and fragment), providing the highest possible signal-to-noise ratio for detecting trace targets in dense tissue matrices.
Demo Report
To intuitively demonstrate the analytical power and superior resolution of our spatial imaging technology, this Demo Report features a sagittal section of a mouse brain as a primary showcase.
Figure 1. A sagittal section of a mouse brain visualized using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI). This three-color overlay image shows the co-localization and distinct distributions of three proteins across the tissue.
Applications
- Cancer Research: Mapping tumor microenvironments to identify biomarkers or therapeutic targets.
- Neuroscience: Examining protein localization in specific brain regions or neuronal structures. For instance, researchers can spatially map trending biomarkers for neurodegeneration (such as Amyloid-β, Tau, and α-synuclein), or precisely visualize neuroinflammation and structural injury using key targets like GFAP, Iba1, TREM2, and NfL.
- Drug Discovery: Investigating the impact of drug treatments on protein expression in tissues.
- Pathology: Studying disease-related protein changes in tissues at the molecular level.
Why Choose Our Targeted Service?
- Unmatched Sensitivity: By narrowing the scope, we can optimize conditions for your specific targets, often achieving better signal-to-noise ratios than massive discovery panels.
- No Antibody Constraints (Antibody-Free): Bypass the limitations of antibody availability and cross-reactivity. Our MS-based approach detects proteins based on their unique mass signatures (peptides), allowing you to target novel isoforms, mutated proteins, or species-specific targets for which no commercial antibodies exist.
- Cost-Efficiency: Pay for the data you actually need. This service is ideal for validation studies following a broad discovery phase.
- Expert Interpretation: You don't just get a spreadsheet; you get a biological narrative supported by our team of pathologists and proteomic specialists.
FAQs
How many proteins can I include in my custom panel?
Our platform is highly flexible. Our platform offers industry-leading flexibility for spatial proteomics. While routine validation panels typically range from 5 to 20 targets to maximize throughput and sensitivity, our mass spectrometry technology can scale to 100+ targets in a single multiplexed panel.
Do you provide raw data or processed images?
Both. You will receive raw image files (TIFF/QPTIFF), processed spatial maps, and a quantitative report (Excel/CSV) detailing protein expression per cell or per region of interest (ROI).
What is the minimum tissue size required?
We can work with samples as small as a 2mm punch biopsy, though larger sections provide better architectural context.
What is the typical lead time for a project?
Our standard turnaround time is 6–8 weeks from the date of sample receipt and project confirmation.
What is the spatial resolution of the platform?
Our service offers flexible resolution settings ranging from 5 to 100 μm. This allows you to tailor the acquisition to your specific research needs, whether you require high-resolution subcellular insights or broader tissue-level mapping.
Can this data be integrated with other spatial omics modalities?
Yes. Our spatial proteomics service is designed for multimodal integration. The resulting data can be used for joint analysis with other spatial datasets, including spatial metabolomics and spatial transcriptomics, providing a comprehensive, multi-layered view of the tissue microenvironment.
Learn about other Q&A.
Sample Preparation Guidelines
To ensure the highest data quality, please adhere to the following preparation standards:
| Feature | Requirement |
|---|---|
| Tissue Type | FFPE (Formalin-Fixed Paraffin-Embedded) or Fresh Frozen. |
| Section Thickness | 5-10 μm (Standard) |
| Slide Type | Positively charged, adhesive slides (e.g., Indium Tin Oxide (ITO) Coated Conductive Slides). |
| Fixation | 10% Neutral Buffered Formalin (NBF) for 12–24 hours for FFPE. |
| Shipping | FFPE at room temp; Frozen samples on dry ice. |
Note: Please contact us prior to sample preparation to determine the most suitable preparation protocol and SOP, in order to avoid sample loss.
