Flow Cytometry-based Sorting (FACS / Flow Sorting) Service
Our Flow Cytometry-based Sorting Service provides high-throughput, precise isolation of specific cell populations from complex and heterogeneous samples, creating a strong foundation for downstream single-cell omics analysis. Using advanced fluorescence-based detection and multiparametric sorting strategies, this service enables the enrichment of rare, viable, and phenotypically defined cell subsets based on distinct surface or intracellular markers. By improving sample purity and reducing background noise, flow cytometric sorting enhances the quality and resolution of subsequent single-cell workflows, including single-cell RNA sequencing, single-cell ATAC-seq, single-cell multi-omics, immune repertoire profiling, and other high-content analyses. This service is especially valuable for applications in immunology, cancer research, stem cell biology, and developmental studies, where accurate isolation of target populations is essential for revealing cellular heterogeneity, lineage relationships, and functional states at single-cell resolution.
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- Our service
- Workflow
- Downstream applications
- Advantages
- Why choose
- FAQs
- Sample preparation
What We Provide
- High-Resolution Sorting: Isolate cells based on multiple parameters such as size, granularity, and specific surface markers.
- Multiple Markers Detection: Utilize fluorescently tagged antibodies for the detection of various cellular characteristics.
- Cell Viability Analysis: Ensure the integrity and viability of sorted cells for downstream applications.
- Customized Protocols: Tailored sorting protocols based on client-specific requirements and experimental needs.
Workflow
What downstream applications are suitable for the sorted cells?
- Single-Cell RNA Sequencing (scRNA-seq)
- Single-Cell ATAC Sequencing (scATAC-seq)
- Single-Cell Multiome (scRNA-seq + scATAC-seq)
- Single-Cell Immune Profiling
- Single-Cell Proteomics
Advantages
- High Purity & Viability: Gentle sorting ensures functional cells are preserved
- Multiparametric Capability: Sort complex populations based on multiple markers simultaneously
- Customized Solutions: Tailored sorting strategies for rare or challenging cell types
- Expert Support: Experienced staff optimize protocols for maximal experimental success
Why Choose Us
- Advanced FACS instruments with high-speed, precision sorting
- Comprehensive consultation and protocol optimization
- Strict quality control for sample integrity and reproducibility
- Efficient sample handling and prompt delivery
- Experienced team supporting complex, multi-parameter sorting
FAQs
What cell types can you sort?
We can sort a wide range of cells, including immune cells, stem cells, cancer cells, and primary cells. Our platform supports both human and animal cells, and we can work with freshly isolated or cryopreserved samples. If your target cells are very rare or have specific characteristics, we can help design a customized sorting strategy to maximize recovery and purity.
What is the minimum cell number required?
We generally recommend starting with a minimum of 1x10^5 cells. Final requirements will vary based on panel complexity, sorting speed, and your desired post-sort yield. Please adjust your starting cell quantity based on the expected proportion of your target cells. For example, if your target cell population constitutes less than 1%, we recommend increasing your starting quantity to 10^6 cells or more.
Can sorted cells be used for functional assays?
Yes, provided they are sorted using surface markers, as we optimize these conditions to maintain the high viability needed for live-cell functional experiments like proliferation, cytotoxicity, or differentiation. However, it is important to note that if your experiment requires intracellular staining, the necessary fixation and permeabilization processes will result in cell death. Consequently, cells sorted via intracellular markers cannot be cultured or used for live functional assays.
Can you sort cells based on intracellular markers?
Yes. We can sort cells based on intracellular proteins, transcription factors, or cytokines using validated fixation and permeabilization protocols. Sorting for intracellular markers requires careful optimization to preserve both fluorescence signal and cell integrity. While intracellular staining may slightly reduce viability compared to surface staining, our protocols maximize cell recovery for functional studies whenever possible.
Learn about other Q&A.
Sample Preparation
- Sample Types: Peripheral blood, bone marrow, tissue dissociates, cultured cells
- Requirements: Single-cell suspension, viable cells, appropriate storage and transport conditions
- Transport: Samples should be kept cold (2-8℃) and delivered promptly to maintain viability
Our team can provide detailed sample preparation guidelines upon request to ensure optimal sorting outcomes. Please contact us for consultation before you start.
