BD Rhapsody Service

BD Rhapsody™ Single-Cell Analysis System

The BD Rhapsody™ Single-Cell Analysis System is a state-of-the-art multiomics platform designed to interrogate complex biological samples at the resolution of individual cells. Utilizing a gentle, microwell-based capture technology, the system efficiently partitions thousands to tens of thousands of single cells into individual picoliter-sized wells alongside uniquely barcoded magnetic beads. A distinct advantage of this platform is its integrated imaging scanner, which allows for real-time visual quality control of cell viability and capture rates before committing to downstream library preparation, ensuring maximum data integrity and cost-efficiency for your projects.Beyond standard gene expression profiling, the BD Rhapsody™ platform excels in highly multiplexed, multiomic applications. It enables the simultaneous measurement of the whole transcriptome (or highly sensitive targeted RNA panels), cell-surface protein expression via BD® AbSeq, and adaptive immune repertoires (TCR/BCR) within the exact same cell. By capturing these multiple layers of biological information at once, the platform provides our clients with unparalleled insights into cellular heterogeneity, making it an ideal choice for identifying rare cell populations and advancing research in oncology, immunology, and complex disease biology.

Our facility is equipped with the complete BD Rhapsody™ infrastructure, combining precise hardware, scalable consumables, and robust bioinformatics to ensure your single-cell experiments succeed from capture to analysis.

Our End-to-End Workflow

Advantages of BD Rhapsody™

Unlike traditional droplet-based systems, the BD Rhapsody™ utilizes a microwell-based partitioning technology that offers superior performance for challenging samples.

• Gentle Capture for Fragile Cells: Gravity-based settling minimizes cellular stress, making it the highly recommended for sensitive types like neutrophils, granulocytes, and stem cells.
• Visual Quality Control: Our integrated BD Rhapsody™ Scanner provides real-time imaging of cell capture and bead loading. We can assess cell viability and multiplet rates before you invest in expensive sequencing.
• Sample Multiplexing: Reduce batch effects and costs by pooling up to 12 samples in a single cartridge using the BD® Single-Cell Multiplexing Kit.
• Bead Archiving & Sub-sampling: Your precious samples are captured on magnetic beads that can be stored for up to 12 weeks. This allows you to "sub-sample" your library—sequencing a portion now and saving the rest for future targeted panels.

Key Applications

• Single-Cell RNA Sequencing: Identifying gene expression profiles at a single-cell level.
• Multi-Omics: Integrating RNA-seq with protein markers for deeper insights.
• Immune Profiling: Analyzing immune cell subsets and responses.
• Tumor Immunology: Studying cancer immunology and therapeutic responses.

Why Choose Us as Your Single-Cell Partner?

• End-to-End Expertise: We guide you from initial experimental design and sample prep all the way through to final data interpretation.
• Dedicated Bioinformatics Support: We deliver clean, pre-processed data and interactive visualization files, not just raw FASTQ files.
• Customization & Flexibility: We can design custom targeted gene panels and integrate proprietary antibodies tailored specifically to your research goals.
• Commitment to Quality: We enforce strict QC metrics at every step—from initial cell viability to final sequencing—to guarantee high-fidelity data.

Our Service Offerings

Need tailored advice? Our technical team is ready to provide personalized consulting to support your specific project needs.