Spatial Lipidomics Service

While spatial metabolomics provides a broad view of all small-molecule metabolites within a tissue, Spatial Lipidomics zeroes in on one of the most structurally diverse and biologically critical classes of molecules: lipids. Lipids are not merely energy storage molecules; they are the fundamental building blocks of cellular membranes and potent signaling molecules that dictate cellular function, disease progression, and tissue architecture.

Because lipids possess unique biochemical properties, they require highly specialized extraction, matrix application, and ionization techniques distinct from general water-soluble metabolites. Our Spatial Lipidomics Service allows researchers to visualize the precise in situ distribution of lipid classes—such as phospholipids, sphingolipids, glycerolipids, and sterols—without losing the critical spatial context of the tissue microenvironment.

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overview of Digital & Molecular Pathology Service

Our services
Workflow
Platform
Applications
Why Choose
FAQs

Our Services

Comprehensive Lipid Profiling & Quantification

Whether you are exploring unknown tissue microenvironments or validating specific lipid targets, our core analytical workflows provide the exact depth of coverage your study requires:

Spatial Untargeted Lipidomics Service: Broad-spectrum discovery mapping of hundreds of known and unknown lipid species, ideal for biomarker identification and hypothesis generation.
Spatial Targeted Lipidomics Service: High-sensitivity, focused imaging of predefined lipid panels (e.g., ceramides, prostaglandins) to precisely validate your target molecules.
Quantitative Spatial Lipidomics Service: Absolute quantification of localized lipid concentrations utilizing precise isotopic internal standards.

Advanced Spatial Imaging Platforms

We leverage industry-leading ionization technologies to match the spatial resolution and sample preservation needs of your specific tissue type:

MALDI Imaging Lipidomics Service: High-throughput, highly sensitive lipid mapping utilizing matrix formulations specifically optimized for diverse lipid polarities.
DESI-Based Lipid Imaging Service: Ambient ionization that requires minimal sample preparation, preserving delicate tissue integrity for downstream H&E or IHC staining.
Secondary Ion Mass Spectrometry (SIMS) High-Resolution Imaging Service: Ultra-high, sub-cellular to nanometer-scale resolution for mapping localized membrane and organelle lipid dynamics.

Bioinformatics & Disease Modeling

Transforming raw spatial maps into biologically and clinically meaningful insights is at the core of our data pipeline:

Disease vs Normal Comparative Spatial Lipidomics: Robust statistical mapping that contrasts healthy and pathological tissues to identify spatially defined disease biomarkers.
Lipid Metabolic Pathway Mapping Service: Seamless integration of spatial data with advanced bioinformatics to visualize active lipid biosynthesis and degradation pathways directly across the tissue architecture.

Workflow

spatial lipidomics workflow

Platform

We utilize a fleet of state-of-the-art mass spectrometry imaging platforms and software dedicated to lipidomic analysis:

High-Resolution Mass Spectrometers: Orbitrap and TOF/TOF systems capable of distinguishing complex lipid isobars and isotopes.
Imaging Sources: Advanced MALDI, DESI, and TOF-SIMS systems.
Data Analysis Software: Industry-leading platforms and proprietary lipid databases to ensure accurate annotation of complex lipid species directly from tissue spectra.

Applications

Neuroscience: Mapping white and gray matter lipid profiles, myelin sheath composition, and lipid alterations in neurodegenerative diseases.
Oncology: Investigating the altered lipid metabolism of the tumor microenvironment, mapping hypoxic tumor cores, and identifying lipid-based therapeutic targets.
Cardiovascular Disease: Highlighting the spatial distribution of cholesterol, cholesteryl esters, and oxidized lipids within atherosclerotic plaques.
Pharmacology & Toxicology: Observing drug distribution and its localized phenotypic effect on tissue lipidomes to assess drug efficacy and toxicity.

Why Choose Us

Multi-Modal Resolution: From ambient whole-organ DESI imaging to sub-cellular SIMS mapping, we tailor the spatial resolution to your specific biological question.
Rigorous Spatial Quantification: We employ cutting-edge techniques for internal standard deposition, overcoming the "ion suppression" challenges common in complex lipid analysis.
End-to-End Bioinformatics: We transform complex, terabyte-sized datasets into clear biological insights, providing pathway mapping and comparative statistical reports.
Complimentary Consultation Service: We provide one-on-one, doctoral-level professional consultation, ensuring the delivery of accurate services.

FAQs

How does this service differ from your spatial metabolomics service?

Spatial metabolomics covers all small molecules (sugars, amino acids, nucleotides), often requiring different ionization polarities and matrices. Our Spatial Lipidomics Service uses highly specialized protocols optimized only for lipid extraction and ionization. Because lipids dominate cell membranes and dictate tissue architecture, this specialized focus yields a much deeper, more accurate coverage of lipid isomers and sub-classes that would be missed in a generalized metabolomics scan. Clients may select the appropriate service based on the specific requirements of their project.

What type of samples do you accept?

We highly recommend fresh-frozen, non-embedded tissue blocks. Please consult our technical team before shipping.

How long does a typical project take?

Standard turnaround time is typically 3 to 4 weeks from sample receipt to the delivery of the final report, depending on the number of samples and the complexity of the imaging resolution requested.

Learn about other Q&A.

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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