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Citric Acid Analysis Service

Citric acid Analysis Service

Citric acid is a  weak organic tribasic acid and it occurs naturally in citrus fruits. Citric acid is an important  intermediate in the Citric acid cycle, which is a series of chemical reactions used by all aerobic organisms to  generate energy in biochemistry. Citric  acid exists in greater amounts in a variety of fruits and vegetables,  especially in citrus fruits. For example, lemons and limes have high  concentrations of the acid and it can constitute as much as 8% of the dry  weight of these fruits.

Scientists at Creative Proteomics utilize a highly quantitative method with  high-performance liquid chromatography (HPLC)  for the determination of Citric Acid levels in various samples, including  Plant, Tissue, and more. High-Performance Liquid Chromatography (HPLC) using a differential refractive  index detector (RID) for the determination of Citric acid levels in a lot of  biological samples. This Methodology provides accurate, reliable, and  reproducible results of Citric acid measurement, which enables us to analyze of  Citric acid levels in vitro and in vivo.

Citrate is an important intermediate in the Citric acid cycle, which is a central  metabolic pathway for animals, plants and bacteria to generate energy through  the oxidation in the form of guanosine triphosphate. Citrate synthase can  catalyzes the formation of citrate from oxaloacetate with acetyl CoA, and then  citrate acid can act as the substrate for aconitase and is converted into  aconitic acid. The Citric acid cycle  ends accompanying with the regeneration of oxaloacetate. This series of  chemical reactions is the source of two-thirds of the food-derived energy in  higher organisms and Hans Adolf Krebs, who is a German-born Britishphysician  and biochemist received the 1953 Nobel Prize in Physiology or Medicine for the  discovery.

The Russian-Polish botanist M. Tswett is  generally recognized as the first person to establish the principles of  chromatography. In a paper he presented in 1906, Tswett described how he filled  a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of  chlorophyll to flow through the chalk, separated the chlorophyll into layers of  different colors. He called this technique “chromatography”. Fundamentally,  chromatography is a technique used to separate the components contained in a  sample. High Performance Liquid  Chromatography (HPLC) is a method able to separate non-volatile, thermally  unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide  application range and quantitative accuracy, is regarded as an indispensable  analytical technique, particularly in the field of organic chemistry. It is  also widely used as a preparation technique for the isolation and purification  of target components contained in mixtures.

Citric acid Analysis Service at Creative Proteomics supports your research in Citric acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample Type
Plant,  Tissue, and more

Method
High-Performance  Liquid Chromatography (HPLC) using a  differential refractive index detector (RID) for the determination of Citric acid levels in a lot of  biological samples. This Methodology provides accurate, reliable, and  reproducible results of Citric acid measurement, which enables us to analyze of Citric acid levels in vitro and in vivo.

Send us your samples, you will get all information that you  need!

* For Research Use Only. Not for use in diagnostic procedures.
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