Plant Hormone Analysis Service

Plant hormones (phytohormones) are long  time known as important components of signaling cascades in plant development  and plant responses to various abiotic and biotic challenges. Plants, unlike  animals, lack glands that produce and secrete hormones. Instead, each cell is  capable of producing hormones, which occur in extremely low concentrations. To  date, a total of 10 structurally unrelated small molecules (abscisic acid  [ABA], auxin [AUX], brassinosteroids [BRs], cytokinins [CKs], ethylene [ETH],  GAs, jasmonates [JAs], nitric oxide, salicylic acid [SA], and strigolactones  [SLs]) have been recognized as plant hormones.

Plant hormones  classificationFigure 1 Plant hormones

Accumulating evidence suggests that  multiple plant hormones often mediate the same biologic process by additive,  synergistic, or antagonistic actions, whereas each type of hormone has a characteristic  biologic effect. For example, plant-pathogen interactions result in changes in  the level of various phytohormones. SA, JA, and ethylene are well-known signal  molecules in plant immunity. Although auxin has a pivotal function in plant  development and growth, this hormone also has a position in plant-pathogen interactions. ABA can positively regulate disease in  some conditions and promote defense response in other cases by antagonistic  interaction with SA and JA/ethylene or synergistic interaction with JA.

The nonpeptide plant hormones are  structurally unrelated small molecules. Plant hormones act as signal molecules  in biological activities and frequently occur in low concentration. The  homeostasis of these hormones is tightly controlled between the biosynthetic  and metabolic pathways. The metabolism of nonpeptide plant hormones is  generally categorized into three types of reactions: hydroxylation, oxidation,  and conjugation. For example, hydroxylation of JA results in partial  biologically active 12-OH-JA and hydroxylation of ABA generates biologically  active 7'-OH ABA, 8'-OH ABA, and 9'-OH ABA. Cytokinin can be inactivated by oxidation.  The formation of hormone conjugates may generate different forms of active  hormones, inactive storage hormones, or intermediates for catabolism, such as  the active JA-isoleucine (Ile) and methyl JA (MeJA), the inactive storage  IAA-alanine, and the intermediate IAA-aspartic acid (Asp). A tiny or small  amount of variation in the concentration of a phytohormone may change  physiologic activity, although the roles of these hormones in different  biologic processes still remain to be elucidated. Thus, measurement of the  level of hormones and hormone metabolites is frequently used in the study of  the molecular regulations of different biologic processes.

Low hormones concentrations in plants and  the presence of compounds that interfere with hormones detection make the  quantitative analysis of hormones a challenge for analytical chemistry. Chromatographic  methods have been used increasingly to exclude interfering compounds, separate different  hormones from each other, and increase the precision of hormones quantification.  The combination of GC or LC with MS has been a major advance in the analysis of  hormones. Here Creative Proteomics established a HPLC-MS/MS-based platform for  quantitative analysis of plant hormones. This platform is sensitive and  reliable for simultaneous quantification of multiple analytes in a small amount  of tissue.



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Plant Hormones Quantified in This Service

With integrated set of  separation, characterization, identification and quantification systems  featured with excellent robustness & reproducibility, high and  ultra-sensitivity, Creative Proteomics provides reliable, rapid and  cost-effective Plant Hormones targeted  metabolomics services.


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