Salicylic Acid Analysis Service

Creative Proteomics offers advanced platforms and services for salicylic acid analysis, including High-Performance Liquid Chromatography (HPLC) for precise quantification and Liquid Chromatography-Mass Spectrometry (LC-MS) for sensitive and comprehensive profiling. Our services include salicylic acid quantification, pathway analysis for plant immune responses, and customized method development tailored to specific research needs, catering to diverse sample types such as plant tissues, biological fluids, and tissue extracts.

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What is Salicylic Acid?

Salicylic acid is a monohydroxybenzoic acid, a phenolic and beta-hydroxy acid with the chemical formula C7H6O3, usually derived from the metabolites of salicin. It has low solubility in water, but can be dissolved by a variety of solvents such as ethanol, ether and chloroform.

Salicylic acid is often used as an exfoliating and anti-inflammatory ingredient in cosmetic products due to its ability to penetrate pores and exfoliate dead skin cells, thereby helping to clear clogged pores and minimize acne and other skin problems.

Salicylic acid is also widely used in medicine, for example as one of the main ingredients in some medications for its antipyretic and anti-inflammatory properties. In agriculture and food science, salicylic acid is used to improve the post-harvest quality of fruits, extending their shelf life by regulating parameters such as respiration rate, weight loss, and degree of softening.

Salicylic acid analysis services have important applications in a variety of fields, from cosmetics to pharmaceuticals to agriculture, where the accuracy of the analytical method is critical to scientific research and product development, and Creative Proteomics offers salicylic acid analysis services to meet the needs of a wide range of sample types.

Salicylic Acid Analysis Services Offered by Creative Proteomics

Salicylic Acid Quantitative Analysis: Determination of salicylic acid concentrations in biological samples, including plants, tissues, and complex matrices.

Salicylic Acid Pathway Analysis: Mapping the role of salicylic acid in plant immune responses and systemic acquired resistance (SAR).

Customized Method Development: Designing unique protocols to meet your specific research objectives.

Technology Platforms for Salicylic Acid Analysis

High-Performance Liquid Chromatography (HPLC)

HPLC offers unmatched precision and reproducibility.

Column Specifications: We utilize the XBridge C18 column (2.5 μm, 4.6 mm × 5 cm) for optimal separation of Salicylic Acid.
Mobile Phase: A mixture of 0.1% phosphoric acid aqueous solution and methanol (1:4) ensures effective separation and detection.
Detector: Ultraviolet (UV) absorption at 254 nm enhances the sensitivity for salicylic acid quantification.

Liquid Chromatography-Mass Spectrometry (LC-MS)

For more complex analyses, LC-MS integrates separation and mass-based identification, making it ideal for applications requiring:

Enhanced Sensitivity: Accurate identification of salicylic acid even at trace levels.
Broad Dynamic Range: Suitable for low- and high-concentration samples in both in vitro and in vivo studies.
Detection: Electrospray ionization (ESI) for precise mass spectrometry.

Sample Requirements for Salicylic Acid Analysis

Sample Type	Minimum Sample Quantity	Notes
Fresh Plant Tissues	≥100 mg	Immediately freeze with liquid nitrogen after collection. Store at -80°C or ship with dry ice.
Dried Plant Tissues	≥50 mg	Ensure complete drying before shipping to prevent degradation.
Biological Samples	≥500 µL (e.g., plasma or serum)	Store at -80°C; avoid repeated freeze-thaw cycles.
Tissue Extracts	≥300 µL	Specify the extraction solvent used for preparation.
Plant Organs (e.g., leaves, stems, roots)	≥100 mg per organ type	Clearly label organ type to account for variability in SA concentration.

Demo

Overlaid trace chromatograms in MRM mode of 137/93 for salicylic acid in cotyledon samples (A) and 209/59 for jasmonic acid in root samples (B) (G. Segarra et al., Phytochemistry 67, 2006).

FAQ of Salicylic Acid Analysis

Are there any potential interferences that could affect the accuracy of salicylic acid analysis?

Yes, there are certain potential interferences when analyzing salicylic acid in complex matrices, such as plant extracts, biological fluids, or food products. These interferences can arise from the presence of other compounds that might have similar chemical properties or absorb at the same wavelength during UV detection. To mitigate this, we use techniques like isotope internal standards and optimization of chromatographic separation conditions to minimize these matrix effects and enhance the specificity of the analysis. Our LC-MS platform is particularly well-suited to address these challenges by providing mass-based detection for higher precision.

What are the steps involved in preparing the standard solutions for salicylic acid analysis?

To ensure accurate and reliable quantification, we prepare standard solutions with precise concentrations of salicylic acid. The primary standard solution is prepared by dissolving 0.1% (w/v) salicylic acid in methyl alcohol. To calibrate the analysis, we then prepare a series of calibration standards at different concentrations, specifically 2, 6, 10, 20, and 30 µg/mL. These standards are essential for generating the calibration curve used in quantifying salicylic acid concentrations in your samples. This rigorous approach guarantees the accuracy of measurements and corrects for any potential interferences or matrix effects.

How do I submit my samples for salicylic acid analysis?

Submitting samples is easy. After discussing your project with our team, you'll receive detailed instructions for sample preparation, packaging, and shipping. Samples should be properly labeled and stored according to the guidelines provided. We recommend using dry ice or insulated containers to preserve the integrity of your samples during shipping. Once your samples arrive at our laboratory, they will be logged in and assigned to the appropriate analytical method based on your requirements. If you have any questions during the submission process, our team is always available to assist.

Why should I choose LC-MS for analyzing salicylic acid in complex matrices like biological samples?

LC-MS is particularly effective for analyzing salicylic acid in complex matrices such as plasma, serum, or plant extracts. One of the primary benefits of LC-MS is its ability to not only separate salicylic acid but also provide mass-based identification, allowing for highly accurate quantification even at trace levels. This makes LC-MS the preferred method when dealing with samples that may have low concentrations of salicylic acid or when you require both qualitative and quantitative data. The integration of chromatography and mass spectrometry offers enhanced sensitivity, specificity, and reliability in your results.

Why should I choose LC-MS for analyzing salicylic acid in complex matrices like biological samples?

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Salicylic Acid Analysis Case Study

Article

Bioactive Phenolic Compounds in Peach Extracts and Their Antidiabetic, Antioxidant, and Antibacterial Activities

Publications

Here are some publications in proteomics research from our clients:

Fatty Acid and Antioxidant Profile of Eggs from Pasture-Raised Hens Fed a Corn- and Soy-Free Diet and Supplemented with Grass-Fed Beef Suet and Liver. 2022. https://doi.org/10.3390/foods11213404
The Brain Metabolome Is Modified by Obesity in a Sex-Dependent Manner. 2024. https://doi.org/10.3390/ijms25063475
Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly. 2021. https://doi.org/10.1038/s41598-021-81481-4
Anti-inflammatory activity of black soldier fly oil associated with modulation of tlr signaling: A metabolomic approach. 2023. https://doi.org/10.3390/ijms241310634
Methyl donor supplementation reduces phospho‐Tau, Fyn and demethylated protein phosphatase 2A levels and mitigates learning and motor deficits in a mouse model of tauopathy. 2023. https://doi.org/10.1111/nan.12931

More Publications

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

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Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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