Kinetin Analysis Service


Kinetin Analysis Service

Kinetin is a type of cytokinin, which is a class of plant  growth substances, also named as phytohormones that promote cell division in  plant roots and shoots. Kinetin is  isolated as a chemical compound from the autoclaved herring sperm DNA with cell  division-promoting activity by a Swedish-born American plant physiologist  Skoog, who was a pioneer in the field of plant growth regulators, particularly  cytokinins. The molecular formula of Kinetin is C10H9N5O and is slightly soluble in cold water, methanol, ethanol, freely  soluble in dilute aqueous hydrochloric acid or sodium hydroxide.

Scientists at Creative Proteomics utilize a highly quantitative method with  high-performance liquid chromatography (HPLC)  for the determination of Kinetin levels in various samples, including Plant,  Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Kinetin  (254 nm) levels in a lot of biological samples. This Methodology provides  accurate, reliable, and reproducible results of Kinetin measurement, which  enables us to analyze of Kinetin levels in vitro and in vivo.

The name Kinetin means that it has ability to induce cell division when it was present in the  medium. So, Kinetin is mainly used  in conjunction with auxin in plant tissue culture to induce the formation of  callus, and then to promote the regeneration of the shoot tissue from callus.  It was believed that Kinetin is an  artificial chemical compound produced form the deoxyadenosine residues of DNA  degraded when standing for a long time or heated during the process of  isolation and does not exist naturally. However, Kinetin exists naturally in the DNA of cells from almost all  organisms and is proved in 1996. Kinetin is now is mainly used in the field of producing new plants from tissue  cultures.

The Russian-Polish botanist M. Tswett is  generally recognized as the first person to establish the principles of  chromatography. In a paper he presented in 1906, Tswett described how he filled  a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of  chlorophyll to flow through the chalk, separated the chlorophyll into layers of  different colors. He called this technique “chromatography”. Fundamentally,  chromatography is a technique used to separate the components contained in a  sample. High Performance Liquid  Chromatography (HPLC) is a method able to separate non-volatile, thermally  unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide  application range and quantitative accuracy, is regarded as an indispensable  analytical technique, particularly in the field of organic chemistry. It is  also widely used as a preparation technique for the isolation and purification  of target components contained in mixtures.

Kinetin Analysis Service Analysis Service at Creative Proteomics supports your research in Kinetin Analysis. HPLC Based Analysis Service  Platform enable us at Creative  Proteomics offers you a state-of-the-art Analysis Service.

Sample Type
Various  Sample Type

Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Kinetin (254 nm) levels in a lot of biological  samples. This Methodology provides accurate, reliable, and reproducible results  of Kinetin measurement, which  enables us to analyze of Kinetin levels in vitro and  in vivo.

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