Abscisic Acid is also known as abscisin II or dormin and is abbreviated as ABA with the chemical molecular formula is C15H20O4. Abscisic Acid is the most famous plant hormone, which are chemicals that regulate plant growth and also are termed plant growth substances. Abscisic Acid can be degraded into phaseic acid by the enzyme named (+)-Abscisic Acid 8'-hydroxylase.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Abscisic Acid levels in various samples, including Plant, Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Abscisic Acid (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Abscisic Acid measurement, which enables us to analyze of Abscisic Acid levels in vitro and in vivo.
Abscisic Acid can regulate many plants developmental process, for example, bud dormancy, plant responses to environmental stress and plant pathogens and so on. The name of Abscisic Acid is meaning shows its role in the abscission of plant leaves. Abscisic Acid is produced in terminal buds, which lead to slow down the plant growth and derive the leaf primordia to develop scales to protect the dormant buds during the winter. Abscisic Acid also can hamper the division of cells from the vascular cambium, and then by suspending primary and secondary growth in order to adapt the cold season. Abscisic Acid is also found in the roots of plants, in which Abscisic Acid can translocate to the leaves and then rapidly changes the osmotic potential of stomatal guard cells, deriving them to shrink and stomata to close in order to esponse to the decreased soil water potential or other stressed environments.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Abscisic Acid Analysis Service at Creative Proteomics supports your research in Abscisic Acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Various Sample Type
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Abscisic Acid (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Abscisic Acid measurement, which enables us to analyze of Abscisic Acid levels in vitro and in vivo.
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