Indole-3-acetic acid is abbreviated as IAA and belongs to the family of the auxin, which is a class of most common, naturally-occurring plant hormones. Indole-3-acetic acid contains a carboxymethyl substituent and is a derivative of indole, which is an aromatic heterocyclic organic compound with a bicyclic structure. Indole-3-acetic acid is a colorless solid and is soluble in polar organic solvents.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Indole-3-acetic Acid levels in various samples, including Plant, Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Indole-3-acetic acid (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Indole-3-acetic acid measurement, which enables us to analyze of Indole-3-acetic acid levels in vitro and in vivo.
Indole-3-acetic acid can A serves as signaling molecule necessary for regulation the plant growth and development, for example, regulation the cell elongation and cell division and so on. Indole-3-acetic acid can enters the plant cell nucleus and then binds with a protein complex, which is consisted with a Ubiquitin-activating enzyme (also termed as E1 in general), a Ubiquitin-conjugating enzyme (E2), and a Ubiquitin ligase (E3) resulting in the increased of the Ubiquitination of Aux/IAA proteins in order to regulate the activity of ARF. Indole-3-acetic acid also has a role in stress tolerance because Indole-3-acetic acid can inhibit the photorespiratory-dependent cell death in photorespiratory catalase mutants. Indole-3-acetic acid is also can be produced among environmental bacteria in order to inhabit soils, waters, plant and animal hosts. Research about whether Indole-3-acetic acid producing fungi can promote plant growth and protection in sustainable agriculture or not in under investigating.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Indole-3-acetic acid Analysis Service at Creative Proteomics supports your research in Indole-3-acetic acid Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Plant, Tissue and more
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Indole-3-acetic acid (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Indole-3-acetic acid measurement, which enables us to analyze of Indole-3-acetic acid levels in vitro and in vivo.
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