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Home > Services > Metabolomics Service > Targeted Metabolomics > Bile Acids Analysis Service

Bile Acids Analysis Service

Bile Acids Analysis Service

Bile acids are a large family of steroids, which has a carboxyl group in the side chain. They are predominantly found in the bile of mammals and other vertebrates. In the liver, bile acids can conjugate with taurine or glycine and form bile salts. Chenodeoxycholic acid and cholic acid are the most abundant bile acids in human bile and they are the primary bile acids synthesized in the liver. Deoxycholic and lithocholic acids are the major secondary bile acids and they are formed by the action of intestinal bacterial bacteria on primary bile acids.

Metabolism of bile acids plays an important role in maintaining and regulating cholesterol homeostasis. About 500 mg of cholesterol is converted to bile salts every day in a healthy adult human. The major pathway for the synthesis of the bile acids in the liver, starts from the conversion of 7alpha-hydroxycholesterol from of cholesterol, while the alternative pathway of bile salt synthesis begins with the formation of 27-hydroxycholesterol or oxysterol-24-hydroxycholesterol. In extra-hepatic tissues, the intermediates generated in these two pathways can be converted to bile salts. These extra-hepatic conversions play an important role in cholesterol homeostasis.

Also, acting as emulsifiers, bile acids facilitate the digestion of triacylglycerol, make lipids more accessible to pancreatic lipases and promote the intestinal absorption of fats (fat-soluble vitamins included).

Various intermediates in bile acids synthesis and the secondary metabolism of these intermediates make bile acids a rather complex mixture. The differences of these compounds in the mixture rely on the number, positions and stereochemistry of hydroxyl groups and the length of the side chain. Not only the concentrations of bile acids in infants, healthy adults and patients with liver disease would be different; the hydroxyl groups may also be introduced at different positions. The complex nature of the bile acids exist in the sample is the key factor determines the analytical method to be used. The bile acids are rather complex mixture and quantification of bile acids is needed. Simple analytical quantification methods have limitations, so time-consuming LC-MS quantification method is needed for bile acids analysis. With LC-MS, variable mixtures of bile acids in blood, urine, meconium, feces and amniotic fluid can be quantified successfully.

Here Creative Proteomics develop a sensitive and rapid method for the analysis of the 66 bile acids physiological samples by LC-MS.

Platform

  • LC-MS

Summary

  • Two-step solvent extraction. Supernatants are combined, dried, and re-suspended by RPLC. Then the vitamins are quantified by ESI by MRM methods.

Sample Requirement

  • Normal Volume: 200 uL plasma, 100 mg tissue, (2E7) cells, (80 mg–human; 40 mg-rodent) feces
  • Minimal Volume: 100uL plasma, 50 mg tissue, (5E6) cells, Feces (50 mg –human; 30 mg-rodent) feces

Report

  • A detailed technical report will be provided at the end of the whole project, including the experiment procedure, MS instrument parameters.
  • Analytes are reported as uM/ml, while CV's are generally 10%.
  • The name of the analytes, abbreviateon, formula, molecular weight and CAS# would also be included in the report.

Bile Acids Quantified in Our Service

Bile Acids Quantified in Our Service
1 12-Ketochenodeoxycholic acid
2 12-Ketolithocholic acid
3 3-Oxocholic acid
4 6,7-Diketolithocholic acid
5 7-Ketodeoxycholic acid
6 7-Ketolithocholic acid
7 Allocholic acid
8 Alloisolithocholic acid
9 Apocholic acid
10 Chenodeoxycholic acid
11 Chenodeoxycholic acid 24-β-D-glucuronide
12 Chenodeoxycholic aicd-3-glucuronide
13 Cholic acid
14 Cholic acid 3-sulfate
15 Dehydrocholic acid
16 Dehydrolithocholic acid
17 Deoxycholic acid
18 Deoxycholic acid - 3- glucuronide
19 Deoxycholic acid 3-sulfate
20 DHCA
21 Dioxolithocholic acid
22 Glycochenodeoxycholic acid
23 Glycochenodeoxycholic acid 3-sulfate
24 Glycocholic acid
25 Glycocholic acid 3-sulfate
26 Glycodehydrocholic acid
27 Glycodeoxycholic Acid
28 Glycodeoxycholic acid 3-sulfate
29 Glycohyocholic acid
30 Glycohyodeoxycholic acid
31 Glycolithocholic acid
32 Glycolithocholic acid 3-sulfate
33 Glycoursodeoxycholic acid
34 Hyodeoxycholic acid
35 Isodeoxycholic acid
36 Isolithocholic acid
37 Lithocholic acid
38 Lithocholic acid 3-sulfate
39 Lithocholic acid-24-glucuronide
40 Lithocholic acid-3-glucuronide
41 Murocholic acid
42 Norcholic acid
43 Nordeoxycholic acid
44 Norursodeoxycholic acid
45 Tauro- β-muricholic acid
46 Tauro-α-muricholic acid
47 Tauro-ω-muricholic acid
48 Taurochenodeoxycholic acid
49 Taurochenodeoxycholic acid 3-sulfate
50 Taurocholic Acid
51 Taurodehydrocholic acid
52 Taurodeoxycholic Acid
53 Taurodeoxycholic acid 3-sulfate
54 Taurohyocholic acid
55 Taurohyodeoxycholic acid
56 Taurolithocholic acid
57 Taurolithocholic acid 3-sulfate
58 Tauroursodeoxycholic Acid
59 Tauroursodeoxycholic acid 3-sulfate
60 THCA
61 Ursocholic Acid
62 Ursodeoxycholic acid
63 α-Muricholic acid
64 β-Muricholic acid
65 λ-Muricholic acid (hyocholic acid)
66 ω-Muricholic acid

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With integrated a set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective bile acids targeted metabolomics services.


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