ATP is the abbreviation of Adenosine triphosphate, which is the basic unit of intracellular energy transfer. ATP consists of an adenine ring, a ribose sugar and three phosphate groups. The phosphoryl groups, starting with the group closest to the ribose, are named as the alpha (α), beta (β), and gamma (γ) phosphates respectively and the two phosphoanhydride bonds are responsible for the high energy content of this molecule.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of ATP levels in various samples, including Animals, Plants, Microorganisms, Cells and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of ATP (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of ATP measurement, which enables us to analyze of ATP levels in vitro and in vivo.
ATP is the main energy source for the majority of cellular functions, which includes the biosynthesis of DNA, RNA and protein. ATP is also very important to the macromolecules transport across cell membranes, for example, exocytosis and endocytosis. ATP also participates to maintain the cell structure by facilitating assembly and disassembly of elements of the cytoskeleton. ATP also is required for the shortening of actin and myosin filament crossbridges, which is responsible for muscle contraction. ATP can be recognised by purinergic receptors, which might be the most abundant receptors in mammalian tissues as a signalling molecule and is important in both the central and peripheral nervous system of human beings. ATP also can be used as source of phosphate groups in the phosphate transfer reactions of kinases.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
ATP Analysis Service at Creative Proteomics supports your research in ATP Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Animals, Plants, Microorganisms, Cells and more
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of ATP (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of ATP measurement, which enables us to analyze of ATP levels in vitro and in vivo.
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